大蒜油对链脲佐菌素诱导的糖尿病大鼠肝脏中 FNDC5 和鸢尾素敏感性的改善作用。

Ameliorative effects of garlic oil on FNDC5 and irisin sensitivity in liver of streptozotocin-induced diabetic rats.

机构信息

Department of Pharmacology, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.

Department of Anatomy, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.

出版信息

J Pharm Pharmacol. 2021 Apr 27;73(6):824-834. doi: 10.1093/jpp/rgab023.

DOI:10.1093/jpp/rgab023

PMID:33739409

Abstract

OBJECTIVES

This study was aimed to investigate the effects of garlic oil (GO), an important natural constituent used in alleviating diabetes and its complications, on the expression levels of irisin and related genes.

METHODS

Thirty-two rats were divided into four groups: Control, Diabetes-Control, Diabetes+GO 100 mg/kg/day and Control+GO 100 mg/kg/day for 45 days. The measurements included: changes in liver Peroxisome proliferator-activated receptor-gamma-coactivator (PGC)-1α, Fibronectin Type-III-Domain-Containing5 (FNDC5), irisin expression, mRNA expression of p38 and TNF-α (Tumour necrosis factor-α), total-antioxidant-status (L-TAS; S-TAS), total-oxidant-status (L-TOS; S-TOS) in liver and serum, respectively.

KEY FINDINGS

There was a significant reduction in serum levels of irisin and S-TAS and expression of PGC-1α and FNDC5 in liver in Diabetes-control compared to Control-group, while a significant increase in serum levels of fasting blood glucose (FBG) and TOS, also p38 and TNF-α expressions in liver. In Diabetes+GO group, there was a significant increase in serum irisin and S-TAS, also expression of PGC-1α and FNDC5 in liver, while serum FBG, S-TOS levels, and mRNA expression of p38 and TNF-α in liver were decreased compared to Diabetes-control group (P < 0.05).

CONCLUSIONS

GO alleviated the diabetic liver injury by decreasing Oxidative-Stress parameters and regulation PGC-lα, FNDC5, irisin and P38, keeping the balance of TAS/TOS and TNF-α.

摘要

目的

本研究旨在探讨大蒜油（GO）作为一种用于缓解糖尿病及其并发症的重要天然成分，对鸢尾素及其相关基因表达水平的影响。

方法

32 只大鼠随机分为 4 组：对照组、糖尿病对照组、糖尿病+GO100mg/kg/天组和对照组+GO100mg/kg/天组，共 45 天。测量指标包括：肝脏过氧化物酶体增殖物激活受体-γ共激活因子（PGC）-1α、纤维连接蛋白 III 型结构域 5（FNDC5）、鸢尾素表达、p38 和肿瘤坏死因子-α（TNF-α）mRNA 表达、肝和血清中总抗氧化状态（L-TAS；S-TAS）、总氧化状态（L-TOS；S-TOS）的变化。

主要发现

与对照组相比，糖尿病对照组血清鸢尾素和 S-TAS 水平以及肝脏 PGC-1α 和 FNDC5 表达显著降低，而空腹血糖（FBG）和 TOS 血清水平以及肝脏 p38 和 TNF-α 表达显著升高。在糖尿病+GO 组，与糖尿病对照组相比，血清鸢尾素和 S-TAS 水平以及肝脏 PGC-1α 和 FNDC5 表达显著增加，而血清 FBG、S-TOS 水平以及肝脏 p38 和 TNF-α mRNA 表达显著降低（P<0.05）。