International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, PR China.
Proteomics. 2021 May;21(10):e2000196. doi: 10.1002/pmic.202000196. Epub 2021 Apr 19.
Plasticware and glassware used in the sample processing stage could result in different analysis performance for macromolecules, which led to the speculation that a similar effect could happen to small molecules. To confirm the speculation, pooled human plasma sample spiked with and without metabolite standards was prepared with three most commonly used container materials (glass, deactivated glass and polypropylene) supplied by different manufacturers after a two-step liquid-liquid extraction, followed by gas chromatography mass spectrometry (GC-MS) based untargeted and targeted metabolomics. The results showed that both GC-MS-based targeted and untargeted metabolomics could be influenced significantly by not only the container material but also the manufacturing procedures employed by different vendors. As a general guideline, it would be highly practical to use the same type of container from the same manufacturer during a whole project. Our study could be extremely valuable for the fellow researchers when dealing with the sample containers for GC-MS-based untargeted and targeted metabolomics.
在样品处理阶段使用的塑料器皿和玻璃器皿可能会对大分子的分析性能产生不同的影响，这导致人们猜测小分子也可能会受到类似的影响。为了证实这一推测，我们使用三种最常用的容器材料（玻璃、钝化玻璃和聚丙烯），由不同制造商提供，在两步液液萃取后，对添加和未添加代谢物标准品的混合人血浆样品进行处理，然后进行基于气相色谱质谱（GC-MS）的非靶向和靶向代谢组学分析。结果表明，不仅容器材料，而且不同供应商采用的制造工艺都会对基于 GC-MS 的靶向和非靶向代谢组学产生显著影响。作为一般准则，在整个项目中使用同一制造商的同一类型容器是非常实用的。在处理基于 GC-MS 的非靶向和靶向代谢组学的样品容器时，我们的研究对同行研究人员可能具有极高的价值。
