College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China.
Key Laboratory of Molecular Biology and Gene Engineering in Jiangxi Province, College of Life Science, Nanchang University, Nanchang, China.
PLoS Genet. 2021 Mar 19;17(3):e1009461. doi: 10.1371/journal.pgen.1009461. eCollection 2021 Mar.
Long noncoding RNAs (lncRNAs) are emerging as important regulators in plant development, but few of them have been functionally characterized in fruit ripening. Here, we have identified 25,613 lncRNAs from strawberry ripening fruits based on RNA-seq data from poly(A)-depleted libraries and rRNA-depleted libraries, most of which exhibited distinct temporal expression patterns. A novel lncRNA, FRILAIR harbours the miR397 binding site that is highly conserved in diverse strawberry species. FRILAIR overexpression promoted fruit maturation in the Falandi strawberry, which was consistent with the finding from knocking down miR397, which can guide the mRNA cleavage of both FRILAIR and LAC11a (encoding a putative laccase-11-like protein). Moreover, LAC11a mRNA levels were increased in both FRILAIR overexpressing and miR397 knockdown fruits, and accelerated fruit maturation was also found in LAC11a overexpressing fruits. Overall, our study demonstrates that FRILAIR can act as a noncanonical target mimic of miR397 to modulate the expression of LAC11a in the strawberry fruit ripening process.
长链非编码 RNA（lncRNAs）在植物发育中作为重要的调控因子而出现，但在果实成熟过程中，它们的功能特征很少被研究。在这里，我们根据多聚腺苷酸化（poly(A)-） depleted 文库和 rRNA depleted 文库的 RNA-seq 数据，从草莓成熟果实中鉴定出了 25613 个 lncRNAs，它们大多数表现出明显的时间表达模式。一个新的 lncRNA，FRILAIR 含有高度保守的 miR397 结合位点，在不同的草莓物种中。FRILAIR 的过表达促进了 Falandi 草莓的果实成熟，这与敲低 miR397 的结果一致，miR397 可以指导 FRILAIR 和 LAC11a（编码一种假定的漆酶 11 样蛋白）的 mRNA 切割。此外，FRILAIR 过表达和 miR397 敲低果实中的 LAC11a mRNA 水平均增加，并且在 LAC11a 过表达果实中也发现了果实成熟加速。总的来说，我们的研究表明，FRILAIR 可以作为 miR397 的非典型靶标模拟物，调节草莓果实成熟过程中 LAC11a 的表达。
