Pharmacology Research Laboratory, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Sector 14, 160014, Chandigarh, India.
Food and Nutritional Biotechnology Laboratory, National Agri-Food Biotechnology Institute, SAS Nagar, Punjab, 140306, India.
Metab Brain Dis. 2021 Aug;36(6):1315-1330. doi: 10.1007/s11011-021-00693-9. Epub 2021 Mar 19.
The night shift paradigm induces a state of chronic partial sleep deprivation (CPSD) and enhances the vulnerability to neuronal dysfunction. However, the specific neuronal impact of CPSD has not been thoroughly explored to date. In the current study, the night shift condition was mimicked in female Swiss albino mice. The classical sleep deprivation model, i.e., Modified Multiple Platform (MMP) method, was used for 8 h/day from Monday to Friday with Saturday and Sunday as a weekend off for nine weeks. Following nine weeks of night shift schedule, their neurobehavioral profile and physiological parameters were assessed along with the activity of the mitochondrial complexes, oxidative stress, serotonin levels, and inflammatory markers in the brain. Mice showed an overall hyperactive behavioral profile including hyperlocomotion, aggression, and stereotyped behavior accompanied by decreased activity of mitochondrial enzymes and serotonin levels, increased oxidative stress and inflammatory markers in whole brain homogenates. Collectively, the study points towards the occurrence of a hyperactive behavioral profile akin to mania and psychosis as a potential consequence of CPSD.
夜班模式会导致慢性部分睡眠剥夺（CPSD），并增加神经元功能障碍的易感性。然而，迄今为止，CPSD 对神经元的具体影响尚未得到彻底探讨。在本研究中，模拟了雌性瑞士白化小鼠的夜班模式。使用经典的睡眠剥夺模型，即改良多平台（MMP）方法，从周一到周五每天进行 8 小时的剥夺，周六和周日休息两天，共进行九周。经过九周的夜班时间表后，评估了它们的神经行为特征和生理参数，以及脑内线粒体复合物的活性、氧化应激、5-羟色胺水平和炎症标志物。结果显示，小鼠表现出总体的多动行为特征，包括过度活跃、攻击和刻板行为，同时伴有线粒体酶活性降低、5-羟色胺水平降低、氧化应激和炎症标志物增加。总的来说，该研究表明，类似于躁狂症和精神病的多动行为特征可能是 CPSD 的潜在后果。
