Barshop Institute for Longevity and Aging Studies, San Antonio, TX, USA.
Department of Medicine - Diabetes, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Adv Exp Med Biol. 2021;1316:1-24. doi: 10.1007/978-981-33-6785-2_1.
Lipids are the critical components of cellular and plasma membrane, which constitute an impermeable barrier of cellular compartments, and play important roles on numerous cellular processes including cell growth, proliferation, differentiation, and signaling. Alterations in lipid metabolism have been implicated in the development and progression of cancers. However, unlike other biomolecules, the diversity in the structures and characteristics of lipid species results in the limited understanding of their metabolic alterations in cancers. Lipidomics is an emerging discipline that studies lipids in a large scale based on analytical chemistry principles and technological tools. Multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) uses direct infusion to avoid difficulties from alterations in concentration, chromatographic anomalies, and ion-pairing alterations to improve resolution and achieve rapid and accurate qualitative and quantitative analysis. In this chapter, lipids and lipid metabolism relevant to cancer research are introduced, followed by a brief description of MDMS-SL and other shotgun lipidomics techniques and some applications for cancer research.
脂质是细胞和质膜的关键组成部分，构成了细胞隔室的不可渗透屏障，并在许多细胞过程中发挥重要作用，包括细胞生长、增殖、分化和信号转导。脂质代谢的改变与癌症的发生和发展有关。然而，与其他生物分子不同，脂质种类的结构和特性的多样性导致人们对其在癌症中的代谢改变的理解有限。脂质组学是一个新兴的学科，它基于分析化学原理和技术工具来大规模研究脂质。基于多维质谱的 shotgun 脂质组学（MDMS-SL）使用直接进样来避免由于浓度变化、色谱异常和离子配对变化引起的困难，以提高分辨率并实现快速、准确的定性和定量分析。本章介绍了与癌症研究相关的脂质和脂质代谢，随后简要描述了 MDMS-SL 和其他 shotgun 脂质组学技术以及它们在癌症研究中的一些应用。
