Division of Cosmetic Science and Technology, Daegu Haany University, Gyeongsan, 38610, Republic of Korea.
Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu, 41940, Republic of Korea.
Biochem Biophys Res Commun. 2021 Apr 30;551:161-167. doi: 10.1016/j.bbrc.2021.02.113. Epub 2021 Mar 16.
Physiological oxygen concentration (physioxia) ranges from 1 to 8% in human tissues while many researchers cultivate mammalian cells under an atmospheric concentration of 21% (hyperoxia). Oxygen is one of the significant gases which functions in human cells including energy production in mitochondria, metabolism in peroxidase, and transcription of various genes in company with HIF (Hypoxia-inducible factors) in the nucleus. Thus, mammalian cell culture should be deliberated on the oxygen concentration to mimic in vivo physiology. Here, we studied if the cultivation of human skin cells under physiological conditions could affect skin significant genes in barrier functions and dermal matrix formation. We further examined that some representative active ingredients in dermatology such as glycolic acid, gluconolactone, and salicylic acid work in different ways depending on the oxygen concentration. Taken together, we present the importance of oxygen concentration in skin cell culture for proper screening of novel ingredients as well as the mechanistic study of skin cell regulation.
生理氧浓度（低氧）在人体组织中范围为 1%至 8%，而许多研究人员在大气氧浓度为 21%（高氧）的条件下培养哺乳动物细胞。氧是在人体细胞中起作用的重要气体之一，包括线粒体中的能量产生、过氧化物酶中的代谢以及与核内 HIF（缺氧诱导因子）一起转录各种基因。因此，哺乳动物细胞培养应考虑氧浓度以模拟体内生理学。在这里，我们研究了在生理条件下培养人类皮肤细胞是否会影响皮肤屏障功能和真皮基质形成的重要基因。我们进一步研究了一些在皮肤病学中具有代表性的活性成分，如甘醇酸、葡萄糖酸内酯和水杨酸，它们根据氧浓度以不同的方式发挥作用。总之，我们提出了在皮肤细胞培养中氧浓度的重要性，以便对新型成分进行适当筛选以及对皮肤细胞调节的机制研究。
