Jordan Jack B, Smallwood Miranda J, Smerdon Gary R, Winyard Paul G
University of Exeter Medical School, Faculty of Life and Health Sciences, University of Exeter, St Luke's Campus, Exeter EX1 2LU, Devon, UK.
DDRC Healthcare, Plymouth Science Park, Research Way, Plymouth PL6 8BU, Devon, UK.
Antioxidants (Basel). 2024 Feb 22;13(3):269. doi: 10.3390/antiox13030269.
The addition of hydrogen peroxide (HO) to cultured cells is widely used as a method to modulate redox-regulated cellular pathways, including the induction of programmed cell death in cell culture experiments and the testing of pro- and antioxidant compounds. Here, we assessed the effect on the cellular response to HO of pre-adapting squamous cell carcinoma cells (A431) to the standard cell culture oxygenation of 18.6% O, compared to cells pre-adapted to a physiological skin O concentration (3.0% O). We showed that cells pre-adapted to 18.6% O resisted HO-induced cell death compared to cells pre-adapted to 3.0% O for 96 h prior to treatment with HO. Moreover, the enzymatic activities of catalase and glutathione reductase, as well as the protein expression levels of catalase, were higher in cells pre-adapted to 18.6% O compared to cells pre-adapted to 3.0% O HO-resistant cells, pre-adapted to 18.6% O, exhibited increased nuclear Nrf-2 levels. It is concluded that A431 cells pre-adapted to standard cell culture oxygenation conditions resist HO-induced cell death. This effect may be related to their heightened activation of Nrf-2.
向培养细胞中添加过氧化氢（HO）被广泛用作调节氧化还原调节细胞途径的方法，包括在细胞培养实验中诱导程序性细胞死亡以及测试促氧化和抗氧化化合物。在此，我们评估了将鳞状细胞癌细胞（A431）预先适应18.6% O的标准细胞培养氧合对细胞对HO反应的影响，并将其与预先适应生理皮肤氧浓度（3.0% O）的细胞进行比较。我们发现，与在HO处理前预先适应3.0% O 96小时的细胞相比，预先适应18.6% O的细胞对HO诱导的细胞死亡具有抗性。此外，与预先适应3.0% O的细胞相比，预先适应18.6% O的细胞中过氧化氢酶和谷胱甘肽还原酶的酶活性以及过氧化氢酶的蛋白表达水平更高。预先适应18.6% O的HO抗性细胞表现出细胞核Nrf - 2水平升高。结论是，预先适应标准细胞培养氧合条件的A431细胞对HO诱导的细胞死亡具有抗性。这种效应可能与其Nrf - 2的激活增强有关。
