氨基酸功能化中空介孔硅立方的设计及其在磷脂酰丝氨酸合成中的应用。
Design of amino-functionalized hollow mesoporous silica cube for enzyme immobilization and its application in synthesis of phosphatidylserine.
机构信息
National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China.
National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China.
出版信息
Colloids Surf B Biointerfaces. 2021 Jun;202:111668. doi: 10.1016/j.colsurfb.2021.111668. Epub 2021 Mar 1.
In this study, hollow mesoporous silica cube (HMSC) modified with amino (-NH) were synthesized and applied in the immobilization of phospholipase D (PLD) via physical adsorption and chemical cross-linking strategy. The amino-functionalized nano carrier HMSC represented excellent immobilization ability and achieved 87.15 % immobilization rate. The immobilized PLD has wider pH application range and thermal stability, and maintained over 90% of the initial activity after incubation at 50 °C for 2 h. After 50 days of storage at 4 ℃, immobilized PLD retained 40.12 % of its initial activity while free PLD lost 88.28% of its initial activity. The modified HMSC with immobilized PLD (HMSC-NH-PLD) retained 50.73% activities after 9 consecutive reuses. Using the HMSC-NH-PLD, a high-efficient method for the conversion of phosphatidylserine (PS) from phosphatidylcholine (PC) and L-serine was proposed. The HMSC-NH-PLD exhibited prominent enzymatic activity for PS synthesis, the maximal conversion of PS was 90.40% with a catalytic efficiency (CE) of 31.95 μmol / (g h under the optimal conditions. The research in this paper provides a sustainable and efficient biocatalysis application for PS synthesis.
在这项研究中，合成了经过氨基（-NH）修饰的中空介孔硅立方体（HMSC），并通过物理吸附和化学交联策略将其应用于磷脂酶 D（PLD）的固定化。氨基功能化的纳米载体 HMSC 表现出优异的固定化能力，达到了 87.15%的固定化率。固定化 PLD 具有更宽的 pH 应用范围和热稳定性，在 50°C 孵育 2 小时后保持初始活性的 90%以上。在 4°C 下储存 50 天后，固定化 PLD 保留了其初始活性的 40.12%，而游离 PLD 失去了其初始活性的 88.28%。经固定化 PLD（HMSC-NH-PLD）修饰的 HMSC 在连续 9 次重复使用后保留了 50.73%的活性。使用 HMSC-NH-PLD，提出了一种从磷脂酰胆碱（PC）和 L-丝氨酸高效转化磷脂酰丝氨酸（PS）的方法。HMSC-NH-PLD 对 PS 合成表现出突出的酶活性，在最佳条件下 PS 的最大转化率为 90.40%，催化效率（CE）为 31.95 μmol/（g·h）。本文的研究为 PS 合成提供了一种可持续、高效的生物催化应用。
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