Division of Laboratory Sciences, National Center for Environmental Health, U.S. Centers for Disease Control and Prevention, Atlanta, GA 30341, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 May 1;1171:122607. doi: 10.1016/j.jchromb.2021.122607. Epub 2021 Feb 27.
We investigated the suitability of isotope-dilution liquid chromatography coupled with tandem mass spectrometry for identifying vitamin E acetate (VEA) in bronchoalveolar lavage (BAL) fluid. This new method demonstrates high accuracy, selectivity, and sensitivity, with mean recoveries higher than 90%, coefficients of variation ranging from 1.5% to 4.5%, and a limit of detection of 1.10 ng/mL. Calibration curves were linear (R > 0.99). The linear range and detection limit of the method were adequate for identifying VEA in 48 of 51 BAL fluid samples collected from people with lung injury resulting from e-cigarettes, or vaping, product use. We conclude that this method is an effective tool for studying VEA accumulation in lungs caused by using e-cigarettes, or vaping, products that contain VEA.
我们研究了同位素稀释液相色谱-串联质谱法在鉴定支气管肺泡灌洗液（BAL）中维生素 E 醋酸酯（VEA）中的适用性。这种新方法具有很高的准确性、选择性和灵敏度，平均回收率高于 90%，变异系数在 1.5%到 4.5%之间，检测限为 1.10ng/mL。校准曲线呈线性（R>0.99）。该方法的线性范围和检测限足以鉴定 51 份来自因电子烟或蒸气产品使用导致肺损伤的人 BAL 液样本中的 VEA。我们得出结论，该方法是研究电子烟或蒸气产品中含有 VEA 导致的 VEA 在肺部蓄积的有效工具。
