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三种不同生活方式改变小鼠模型肝脏中微小RNA的差异表达谱

Differential microRNAs expression profiles in liver from three different lifestyle modification mice models.

作者信息

Gong Huan, Zhang Ming, Han Yiwen, Zhang Ying, Pang Jing, Zhao Yanyang, Chen Beidong, Wu Wei, Qi Ruomei, Zhang Tiemei

机构信息

The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Beijing Hospital, National Center of Gerontology, National Health Commission; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, 100730, People's Republic of China.

Chinese People's Liberation Army General Hospital, Beijing, People's Republic of China.

出版信息

BMC Genomics. 2021 Mar 19;22(1):196. doi: 10.1186/s12864-021-07507-3.

DOI:10.1186/s12864-021-07507-3
PMID:33740891
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7977600/
Abstract

BACKGROUND

MicroRNAs play an important role in many fundamental biological and pathological processes. Defining the microRNAs profile underlying the processes by beneficial and detrimental lifestyles, including caloric restriction (CR), exercise and high-fat diet (HF), is necessary for understanding both normal physiology and the pathogenesis of metabolic disease. We used the microarray to detect microRNAs expression in livers from CR, EX and HF mice models. After predicted potential target genes of differentially expressed microRNAs with four algorithms, we applied GO and KEGG to analyze the function of predicted microRNA targets.

RESULTS

We describe the overall microRNAs expression pattern, and identified 84 differentially expressed microRNAs changed by one or two or even all the three lifestyle modifications. The common and different enriched categories of gene function and main biochemical and signal transduction pathways were presented.

CONCLUSIONS

We provided for the first time a comprehensive and thorough comparison of microRNAs expression profiles in liver among these lifestyle modifications. With this knowledge, our findings provide us with an overall vision of microRNAs in the molecular impact of lifestyle on health as well as useful clues for future and thorough research of the role of microRNAs.

摘要

背景

微小RNA在许多基本生物学和病理过程中发挥重要作用。通过有益和有害的生活方式，包括热量限制（CR）、运动和高脂饮食（HF）来确定这些过程背后的微小RNA谱，对于理解正常生理学和代谢疾病的发病机制都是必要的。我们使用微阵列检测CR、EX和HF小鼠模型肝脏中的微小RNA表达。在用四种算法预测差异表达微小RNA的潜在靶基因后，我们应用基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析预测的微小RNA靶标的功能。

结果

我们描述了整体微小RNA表达模式，并鉴定出84种差异表达的微小RNA，它们因一种、两种甚至所有三种生活方式改变而发生变化。呈现了基因功能以及主要生化和信号转导途径的常见和不同富集类别。

结论

我们首次对这些生活方式改变中肝脏微小RNA表达谱进行了全面而深入的比较。有了这些知识，我们的发现为我们提供了一个关于微小RNA在生活方式对健康的分子影响方面的整体视角，以及未来深入研究微小RNA作用的有用线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/6dbb8deae80e/12864_2021_7507_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/e5840144b5f2/12864_2021_7507_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/f904ddfc468a/12864_2021_7507_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/bc75daab14a5/12864_2021_7507_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/086d5562268c/12864_2021_7507_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/48644718137f/12864_2021_7507_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/9b0386aae0f8/12864_2021_7507_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/6dbb8deae80e/12864_2021_7507_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/e5840144b5f2/12864_2021_7507_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/f904ddfc468a/12864_2021_7507_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/bc75daab14a5/12864_2021_7507_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/086d5562268c/12864_2021_7507_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/48644718137f/12864_2021_7507_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/9b0386aae0f8/12864_2021_7507_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c4/7977600/6dbb8deae80e/12864_2021_7507_Fig7_HTML.jpg

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