College of Chemistry, Green Catalysis Center, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Zhengzhou University, Zhengzhou, 450001, China.
Institute of Chemical Biology and Clinical Application at the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450001, China.
Mikrochim Acta. 2021 Mar 19;188(4):130. doi: 10.1007/s00604-021-04785-2.
A two-dimensional (2D) Co-MOF nanosheet-based nanozyme was developed for colorimetric detection of disease-related biomolecules. The prepared 2D Co-MOFs exhibited ultrahigh peroxidase catalytic activity. 2D Co-MOFs can catalyze the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to the blue product oxTMB, accompanying an obvious change of absorption value at 652 nm. However, alkaline phosphatase can catalyze the hydrolysis of L-ascorbic acid-2-phosphate to produce ascorbic acid which can reduce the oxTMB to TMB, resulting in an obvious color fading. Therefore, by recording the change of absorption value at 652 nm, the 2D Co-MOF nanosheets were used to detect ascorbic acid (AA) and alkaline phosphatase (ALP). The limit of detection for AA and ALP was 0.47 μM and 0.33 U L, respectively. The limit of quantification for AA and ALP was 1.56 μM and 1.1 U L, respectively. The developed nanozyme was successfully used to determine alkaline phosphatase in clinical human serum samples and the results were consistent with those provided by the hospital. Furthermore, by integrating 2D Co-MOF nanosheets with image recognition and data processing function fixed on a smartphone, a portable test of ascorbic acid was reached. Schematic presentation of the preparation of two-dimensional Co-MOF nanosheet-based nanozyme and their application in portable detection of biomolecules.
二维（2D）Co-MOF 纳米片基纳米酶用于疾病相关生物分子的比色检测。所制备的 2D Co-MOF 具有超高过氧化物酶催化活性。2D Co-MOF 可以催化无色 3,3',5,5'-四甲基联苯胺（TMB）氧化为蓝色产物 oxTMB，伴随 652nm 处吸光度的明显变化。然而，碱性磷酸酶可以催化 L-抗坏血酸-2-磷酸水解生成抗坏血酸，抗坏血酸可以将 oxTMB 还原为 TMB，导致明显的颜色褪色。因此，通过记录 652nm 处吸光度的变化，可以使用 2D Co-MOF 纳米片来检测抗坏血酸（AA）和碱性磷酸酶（ALP）。AA 和 ALP 的检测限分别为 0.47μM 和 0.33U L。AA 和 ALP 的定量限分别为 1.56μM 和 1.1U L。所开发的纳米酶成功用于测定临床人血清样品中的碱性磷酸酶，结果与医院提供的结果一致。此外，通过将 2D Co-MOF 纳米片与固定在智能手机上的图像识别和数据处理功能集成，实现了便携式抗坏血酸检测。二维 Co-MOF 纳米片基纳米酶的制备及在生物分子便携式检测中应用的示意图。
