Anhui Laboratory of Molecule-Based Materials; Key Laboratory of Functional Molecular Solids, Ministry of Education; Anhui Key Laboratory of Chemo-Biosensing, School of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, People's Republic of China.
Anhui Meijia New Materials Company Limited, Wuhu, 241000, People's Republic of China.
Mikrochim Acta. 2019 Nov 5;186(12):745. doi: 10.1007/s00604-019-3887-6.
A colorimetric assay is presented for the determination of ascorbic acid (AA). Manganese(II) doped carbon dots (Mn-CDs) were prepared by a convenient hydrothermal route and are shown to possess oxidase-like catalytic ability. They catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by dissolved oxygen to form a blue colored product (oxTMB). AA can reduce blue oxTMB to colorless TMB. The fading of the blue color (measured at 652 nm) can be applied for quantifying AA in the 50 to 2500 nM concentration range and with a 9 nM detection limit. The method was successfully used for the quantitation of AA in real samples. Graphical abstract Schematic representation of a colorimetric assay platform for the sensitive detection of ascorbic acid (AA) in view of inhibitory effect of AA on the 3,3',5,5'-tetramethylbenzidine (TMB) oxidation, benefitting from excellent oxidase-like catalytic activity of manganese(II) doped carbon dots (Mn-CDs).
呈现了一种用于测定抗坏血酸（AA）的比色分析方法。通过简便的水热路线制备了锰（II）掺杂的碳点（Mn-CDs），并证明其具有氧化酶样的催化能力。它们可以催化溶解氧将 3,3',5,5'-四甲基联苯胺（TMB）氧化为蓝色产物（oxTMB）。AA 可以将蓝色 oxTMB 还原为无色 TMB。蓝色的褪色（在 652nm 处测量）可用于定量 50 至 2500nM 浓度范围内的 AA，检测限为 9nM。该方法成功用于实际样品中 AA 的定量。
