用于体内转移能力的多重分析的条形码技术。
Barcoding Technology for Multiplexed Analysis of Metastatic Ability In Vivo.
机构信息
Department of Medical Oncology, West German Cancer Center, University Hospital Essen at the University of Duisburg-Essen, Essen, Germany.
出版信息
Methods Mol Biol. 2021;2294:239-251. doi: 10.1007/978-1-0716-1350-4_17.
DNA barcoding allows the quantitative, biomarker-free tracking of individual cell populations in mixed/heterogeneous cell pools. Here, we describe a multiplexed in vivo screening platform based on DNA barcoding technology to interrogate compound libraries for their effect on metastatic seeding in vivo. We apply next-generation sequencing (NGS) technology to quantitatively analyze high-throughput compound screening in mice. Up to 96 compounds and controls can be screened for their effect on metastatic ability in a single mouse.
DNA 条形码技术可实现对混合/异质细胞群体中单个细胞群体的定量、无生物标志物追踪。在这里，我们描述了一种基于 DNA 条形码技术的多重体内筛选平台，用于研究化合物文库对体内转移定植的影响。我们应用下一代测序 (NGS) 技术对小鼠体内高通量化合物筛选进行定量分析。在一只小鼠中，最多可以筛选 96 种化合物及其对照物，以研究它们对转移能力的影响。
Zotero 插件