Cancer Science Program, Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain.
CIBERONC, Madrid, Spain.
Methods Mol Biol. 2021;2294:253-267. doi: 10.1007/978-1-0716-1350-4_18.
Metastasis is the main cause of death for cancer patients, but our ability to improve clinical outcome first requires a better understanding of the dynamics, cellular mechanisms, and kinetics of metastasis. In prostate cancer (PCa), metastatic tumor cells preferentially colonize to bone. However, a lack of applicable mouse models has limited our ability to study this process accurately. Here, we describe a strategy to bypass this limitation: human PCa cells are injected into immunodeficient mice (at tibia, the left ventricle of heart and the iliac artery). Using this novel technique, the metastatic capabilities of these human PCa cells (e.g., colonization and proliferation potential) can be analyzed in bone with an in vivo imaging system.
转移是癌症患者死亡的主要原因，但要提高临床疗效，首先需要更好地了解转移的动力学、细胞机制和动力学。在前列腺癌（PCa）中，转移性肿瘤细胞优先定植于骨骼。然而，缺乏适用的小鼠模型限制了我们准确研究这一过程的能力。在这里，我们描述了一种克服这一限制的策略：将人 PCa 细胞注射到免疫缺陷小鼠（胫骨、左心室和髂动脉）中。使用这种新技术，可以使用体内成像系统分析这些人 PCa 细胞在骨骼中的转移能力（例如定植和增殖潜力）。
