Department of Stomatology, First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China.
Department of Stomatology, Lianyungang Municipal Oriental Hospital, Lianyungang, Jiangsu, China.
J Oral Pathol Med. 2021 Aug;50(7):681-691. doi: 10.1111/jop.13169. Epub 2021 Apr 18.
Long non-coding RNA (lncRNA) small nucleolar RNA host gene 15 (SNHG15) has been discovered and demonstrated to have significant function in multiple cancers. Nevertheless, how it participates in the progression of oral squamous cell carcinoma (OSCC) and its potential regulatory system are still unclear.
RT-qPCR detected the expression of SNHG15, miR-188-5p, and DAAM1. RNA pull down, RT-qPCR, and bioinformatics were used for finding and selecting downstream targets of SNHG15.
SNHG15 presented a high expression in OSCC cells. Moreover, inhibition of SNHG15 exhibited repressive influence on proliferative, migrated, and invasive abilities but induce apoptosis of OSCC cells. Through the search of bioinformatics and RNA pull down assays, we confirmed that miR-188-5p was one target of SNHG15 in OSCC cells. Additionally, miR-188-5p could hamper the growth of OSCC cells. Moreover, it was manifested that DAAM1 was down-regulated by miR-188-5p. DAAM1 was up-regulated in OSCC cells. Furthermore, it exerted oncogenic function in the course of OSCC. Eventually, overexpression of DAAM1 offsets the effects of down-regulation of SNHG15 on the development of OSCC.
To summarize, our study certified that SNHG15 contributed to the process of OSCC via sponging miR-188-5p to elevate DAAM1 expression. SNHG15 might offer novel sight to improve the results of treatment for OSCC.
长链非编码 RNA（lncRNA）小核仁 RNA 宿主基因 15（SNHG15）已被发现并证明在多种癌症中具有重要功能。然而，它如何参与口腔鳞状细胞癌（OSCC）的进展及其潜在的调控系统仍不清楚。
RT-qPCR 检测 SNHG15、miR-188-5p 和 DAAM1 的表达。采用 RNA 下拉、RT-qPCR 和生物信息学方法寻找和选择 SNHG15 的下游靶标。
SNHG15 在 OSCC 细胞中表达较高。此外，抑制 SNHG15 对 OSCC 细胞的增殖、迁移和侵袭能力具有抑制作用，但诱导 OSCC 细胞凋亡。通过生物信息学和 RNA 下拉实验的搜索，我们证实 miR-188-5p 是 OSCC 细胞中 SNHG15 的一个靶标。此外，miR-188-5p 可以抑制 OSCC 细胞的生长。此外，DAAM1 在 OSCC 细胞中表达下调。此外，它在 OSCC 过程中发挥致癌作用。最终，过表达 DAAM1 抵消了 SNHG15 下调对 OSCC 发展的影响。
总之，我们的研究证实，SNHG15 通过海绵吸附 miR-188-5p 来升高 DAAM1 的表达，促进 OSCC 的发生。SNHG15 可能为改善 OSCC 治疗效果提供新的思路。
