Circ_0058063 通过海绵吸附 miR-145-5p 和上调 SERPINE1 促进口腔鳞状细胞癌的发展。
Circ_0058063 contributes to oral squamous cell carcinoma development by sponging miR-145-5p and upregulating SERPINE1.
机构信息
Department of Stomatology, Henan Provincial People's Hospital, Zhengzhou, China.
出版信息
J Oral Pathol Med. 2022 Aug;51(7):630-637. doi: 10.1111/jop.13331. Epub 2022 Aug 4.
BACKGROUND
We aimed to demonstrate the effects circ_0058063 exerted on oral squamous cell carcinoma (OSCC) and its downstream mechanism associated with miR-145-5p and SERPINE1.
METHODS
The relevant contents of miR-145-5p, circ 0058063, and SERPINE1 mRNAs in OSCC were evaluated using quantitative reverse transcription polymerase chain reaction. Functional experiments including CCK-8, Transwell, Western blot, and in vivo experiment were implemented to investigate the biological impacts on OSCC cells. Using dual-luciferase reporter, RIP, and RNA pull-down assays, the direct binding relationship between miR-145-5p, circ 0058063, and SERPINE1, SMAD3, CYR61, and IGF1R mRNAs was verified.
RESULTS
In OSCC, Circ 0058063 was significantly overexpressed. Knockdown of circ_0058063 suppressed OSCC cell migration and proliferation, but enhanced cell apoptosis. Functionally and mechanistically, circ_0058063 could specifically bind with miR-145-5p and thus upregulated expression of downstream target SERPINE1, which together contributed to the progression of OSCC.
CONCLUSION
Circ_0058063 could promote the malignant behavior of OSCC by upregulating SERPINE1 through sponging miR-145-5p.
背景
本研究旨在探讨 circ_0058063 对口腔鳞状细胞癌（OSCC）的影响及其与 miR-145-5p 和 SERPINE1 相关的下游机制。
方法
采用实时定量逆转录聚合酶链反应（qRT-PCR）评估 OSCC 中 miR-145-5p、circ_0058063 和 SERPINE1 mRNA 的相关含量。通过 CCK-8、Transwell、Western blot 和体内实验等功能实验，研究其对 OSCC 细胞的生物学影响。利用双荧光素酶报告基因、RNA 免疫沉淀（RIP）和 RNA 下拉实验验证 miR-145-5p、circ_0058063 与 SERPINE1、SMAD3、CYR61 和 IGF1R mRNA 之间的直接结合关系。
结果
在 OSCC 中，circ_0058063 明显过表达。circ_0058063 敲低抑制 OSCC 细胞迁移和增殖，但促进细胞凋亡。功能和机制上，circ_0058063 可特异性结合 miR-145-5p，从而上调下游靶基因 SERPINE1 的表达，共同促进 OSCC 的进展。
结论
circ_0058063 通过海绵吸附 miR-145-5p 而上调 SERPINE1，促进 OSCC 的恶性行为。
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