Wang Xiao-Tong, Yang Hong
Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, College of Architectural Engineering, Beijing University of Technology, Beijing 100124, China.
Huan Jing Ke Xue. 2021 Apr 8;42(4):1930-1938. doi: 10.13227/j.hjkx.202009248.
In order to clarify the characteristics of anaerobic ammonia oxidizing (ANAMMOX) sludge and the succession rule of bacteria based on particle size differentiation, the performance change and microbial community structure of ANAMMOX floc sludge during the formation of particles in the reaction system of a high ammonia-nitrogen biofilter were studied. The results indicated that the specific activity (SAA) and tolerance of the ANAMMOX granular sludge (AnGS) were significantly improved by increasing the particle size, and the SAA of R4(>4.75 mm) was up to 426.8 mg·(g·d), but it also had adverse effects on mass transfer. The results of the high-throughput sequencing showed that dynamic changes between bacterial genera were common. When the particle size was less than 4.75 mm, the increase in particle size strengthened the stability of the bacterial flora, the ammonia oxidizing bacteria (AOB) with more flocs were eliminated, and the nitrogen removal ratio gradually stabilized. R3 (2.8-4.75 mm) exhibited the most specific flora composition, and the functional bacteria accounted for 52.7%, while the R4 community complexity increased. Furthermore, the proportion of functional bacteria decreased, and the abundance of heterotrophic bacteria increased, which negatively affected the particle structure. In addition, the R3 microorganism has the best gene function expression level, which is significantly better than small particles in gene replication repair and energy conversion. Finally, the evolution of AnGS was analyzed through the OTU matrix between the samples. These results have some guiding significance for the optimization of the AnGS system and will be helpful for the application of the ANAMMOX process.
为了阐明厌氧氨氧化（ANAMMOX）污泥的特性以及基于粒径分化的细菌演替规律，研究了高氨氮生物滤池反应系统中颗粒形成过程中ANAMMOX絮状污泥的性能变化和微生物群落结构。结果表明，通过增大粒径，ANAMMOX颗粒污泥（AnGS）的比活性（SAA）和耐受性显著提高，R4（>4.75 mm）的SAA高达426.8 mg·(g·d)，但对传质也有不利影响。高通量测序结果表明，细菌属之间的动态变化很常见。当粒径小于4.75 mm时，粒径的增加增强了细菌群落的稳定性，更多絮体的氨氧化细菌（AOB）被去除，脱氮率逐渐稳定。R3（2.8 - 4.75 mm）表现出最特殊的菌群组成，功能菌占52.7%，而R4群落复杂性增加。此外，功能菌比例下降，异养菌丰度增加，对颗粒结构产生负面影响。另外，R3微生物的基因功能表达水平最佳，在基因复制修复和能量转换方面明显优于小颗粒。最后，通过样本间的OTU矩阵分析了AnGS的进化。这些结果对AnGS系统的优化具有一定的指导意义，将有助于ANAMMOX工艺的应用。
