Zhang Hong-Na, Dong Meng-Jie, Zhou Yu-Fa, Sun Jia-Xin, Chang Mei-Jie, Zhai Zhen-Zhen
College of Biological Science and Engineering, Hebei University of Economics and Business, Shijiazhuang 050061, China.
Animal Husbandry Bureau of Daiyue Region, Tai'an 271000, China.
Huan Jing Ke Xue. 2021 Apr 8;42(4):2080-2088. doi: 10.13227/j.hjkx.202009038.
Increasing antibiotic resistant genes (ARG) are being detected in animal manure-amended soil, causing public concerns. However, the effects of animal manure fertilization on the ARG of vegetable endophytes remain unknown. In this study, high-throughput quantitative PCR (HT-qPCR) was used to explore the effects of poultry manure fertilization on the bacterial community and resistome in the soil, root endophytes, and leaf endophytes from L.. The results indicated that poultry manure fertilization not only enhanced the number of ARG in soil and root endophytes, but also increased the abundance of ARG in the soil, root endophytes, and leaf endophytes. The relationship analysis demonstrated that the ARG profile in the soil and vegetable endophytes was significantly correlated with the bacterial community, and the ARG abundance was highly correlated with Proteobacteria, Acidobacteria, Actinobacteria, and Cyanobacteria. In addition, the overlapping ARG between the L. endophyte and soil indicated the internal spread route of the ARG from the manure to L through the soil. In conclusion, poultry manure fertilization increased the diversity and abundance of the ARG in vegetables via the manure-soil-vegetable pathway.
在施用动物粪便的土壤中，越来越多的抗生素抗性基因（ARG）被检测到，这引起了公众的关注。然而，动物粪便施肥对蔬菜内生菌ARG的影响仍不清楚。在本研究中，采用高通量定量PCR（HT-qPCR）技术，探究了家禽粪便施肥对番茄土壤、根部内生菌和叶片内生菌中细菌群落及抗性组的影响。结果表明，家禽粪便施肥不仅增加了土壤和根部内生菌中ARG的数量，还提高了土壤、根部内生菌和叶片内生菌中ARG的丰度。相关性分析表明，土壤和蔬菜内生菌中的ARG谱与细菌群落显著相关，ARG丰度与变形菌门、酸杆菌门、放线菌门和蓝细菌门高度相关。此外，番茄内生菌与土壤之间的重叠ARG表明了ARG从粪便通过土壤到番茄的内部传播途径。总之，家禽粪便施肥通过粪便-土壤-蔬菜途径增加了蔬菜中ARG的多样性和丰度。
