Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China.
Sci Total Environ. 2022 Apr 15;817:152971. doi: 10.1016/j.scitotenv.2022.152971. Epub 2022 Jan 10.
The threat of antibiotic resistance genes (ARGs) caused by animal manure application to human health has been the focus of attention in agriculture. Applying lime to acid soil for the amelioration of soil acidity is a prevailing agricultural practice. However, the role of lime on the spread of antibiotic resistome from soil to plant is unknown. In this study, a pot experiment of lettuce was established in the acid black soil with lime addition at the rate (w/w) of 0%, 0.08%, 0.16%, and 0.32% of the total soil mass to explore the transmission of ARGs introduced by the fresh poultry manure in the soil-plant system. The bulk and rhizosphere soils as well as the leaf samples were collected after lettuce was cultivated for 60 days, the bacterial community and antibiotic resistome in these samples were determined by using Illumina sequencing and high-throughput quantitative PCR (HT-qPCR) methods, respectively. Results showed that lime application decreased the number and abundance of ARGs and slowed down the spread of manure-derived ARGs in the soil-plant system. The ARGs and bacterial community composition were significantly varied among bulk soils, rhizosphere soils and leaf endophyte, and also influenced by lime within the same sampling types. The structural equation model further demonstrated that the lime addition had a negative effect on ARG diversity, which was also indirectly regulated by bacterial community diversity. These findings suggest that lime addition can alleviate the level and dissemination of ARGs in soils and provide a potential measure to control the spread of ARGs derived from animal manure.
动物粪便施用于土壤会导致抗生素耐药基因（ARGs）威胁人类健康，这一问题一直是农业领域关注的焦点。在酸性土壤中施用石灰以改善土壤酸度是一种常见的农业实践。然而，石灰对土壤中抗生素耐药组从土壤向植物传播的影响尚不清楚。在这项研究中，在酸性黑土中进行了生菜盆栽实验，石灰的添加量分别为土壤总质量的 0%、0.08%、0.16%和 0.32%，以探讨新鲜禽粪在土壤-植物系统中引入的 ARGs 的传播情况。在生菜种植 60 天后，采集了土壤、根际土和叶片样本，使用 Illumina 测序和高通量定量 PCR（HT-qPCR）方法分别测定了这些样本中的细菌群落和抗生素耐药组。结果表明，石灰的施用减少了 ARGs 的数量和丰度，并减缓了粪肥衍生的 ARGs 在土壤-植物系统中的传播。ARGs 和细菌群落组成在原状土、根际土和叶片内生菌之间存在显著差异，并且在同一采样类型中也受到石灰的影响。结构方程模型进一步表明，石灰的添加对 ARG 多样性有负面影响，而这种影响又受到细菌群落多样性的间接调节。这些发现表明，石灰的添加可以减轻土壤中 ARGs 的水平和传播，并为控制动物粪肥衍生的 ARGs 传播提供了一种潜在的措施。
