Department of Physical Therapy, City University of New York - College of Staten Island, USA; Department of Osteology and Biomechanics, University Medical Center Hamburg Eppendorf, DE, Germany.
Department of Physical Therapy, City University of New York - College of Staten Island, USA.
J Biomech. 2021 May 7;120:110341. doi: 10.1016/j.jbiomech.2021.110341. Epub 2021 Mar 10.
The endoskeleton of teleosts (bony fish) includes a vertebral spine with articulating rib bones (RBs) similar to humans and further encompasses mineralized tissues that are not found in mammals, including intermuscular bones (IBs). RBs form through endochondral ossification and protect the inner organs, and IBs form through intramembranous ossification within the myosepta and play a role in force transmission and propulsion during locomotion. Based on previous findings suggesting that IBs show a much higher ability for fracture strain compared to mammalian bones, this study aims to investigate whether this ability is general to teleost bones or specific to IBs. We analyzed RBs and IBs of 25 North Atlantic Herring fish. RBs were analyzed using micro-mechanical tensile testing and micro-computed tomography, and both RB and IB were additionally analyzed with Raman spectroscopy. Based on our previous results from IB, we found that RBs are more elastically deformable (on average, 50% higher yield strain and 115% higher elastic work) and stronger (55% higher fracture stress) than values reported for IBs. However, these differences were neither associated with a higher Young's modulus nor a higher degree of mineralization in RBs. Astonishingly, RBs and IBs showed similar fracture strains (12-15% on average, reaching up to 20%), reflecting a much higher ability for tensile deformation than reported for mammalian bone, and further highlighting the biomimetic potential of teleost fish bones for inspiring innovative biomaterials.
硬骨鱼（骨鱼）的内骨骼包括一个带有关节肋骨（RB）的脊椎，类似于人类，还包括哺乳动物中没有的矿化组织，包括肌间骨（IB）。RB 通过软骨内骨化形成，保护内部器官，IB 通过肌膜内骨化在肌隔内形成，在运动时发挥力传递和推进的作用。基于先前的研究表明，IB 比哺乳动物骨骼具有更高的断裂应变能力，本研究旨在探讨这种能力是普遍适用于硬骨鱼骨骼还是特定于 IB。我们分析了 25 条北大西洋鲱鱼的 RB 和 IB。使用微机械拉伸测试和微计算机断层扫描分析 RB，同时使用 Raman 光谱分析 RB 和 IB。基于我们之前从 IB 获得的结果，我们发现 RB 具有更高的弹性变形能力（平均高出 50%的屈服应变和 115%的弹性功）和更高的强度（高出 55%的断裂应力），高于我们之前报道的 IB 值。然而，这些差异与 RB 中的更高杨氏模量或更高矿化程度无关。令人惊讶的是，RB 和 IB 表现出相似的断裂应变（平均 12-15%，最高可达 20%），反映出比哺乳动物骨骼更高的拉伸变形能力，进一步强调了硬骨鱼骨骼在启发创新生物材料方面的仿生潜力。
