Department of Orthopaedics, Affiliated Hospital of Chengde Medical College, 36 Nanyingzi Street, Chengde, Hebei, 067000, People's Republic of China.
J Orthop Surg Res. 2021 Mar 20;16(1):204. doi: 10.1186/s13018-021-02333-5.
This study was aimed to explore the application value of modified closed biopsy technique in puncture biopsy of rabbit VX2 transplanted bone tumor model.
VX2 tumor was transplanted into the bilateral tibia of 30 rabbits through the tibial plateau to make the model of VX2 transplanted bone tumor. Seven days after modeling, the proximal tibia biopsy was performed under the guidance of X-ray, and the biopsy specimen was examined pathologically. The left leg was biopsied with modified closed biopsy technique (experimental group), and the right leg was biopsied with hollow needle (control group). After 14 days of modeling, all rabbits were killed after X-ray examination around the puncture hole, and the soft tissue around the puncture hole was taken for pathological examination, and the expression levels of PCNA and CD34 in the tissue extract were detected by enzyme-linked immunosorbent assay (ELISA).
By the end of the experiment, a total of 3 rabbits died, and finally, 27 rabbits were included in the study. Tumor cells were detected in all the intramedullary specimens obtained by puncture biopsy. On the 14th day after modeling, X-ray showed that the occurrence rate of periosteal reaction and extraosseous high-density shadow around the puncture hole was 14.81% (4/27) in the experimental group and 40.74% (11/27) in the control group. The difference was statistically significant (P<0.05). The pathological results of soft tissue around the puncture hole showed that the tumor cell metastasis rate was 29.63% (8/27) in the experimental group and 100% (27/27) in the control group, and the difference was statistically significant (P<0.05). The expression levels of PCNA and CD34 in the experimental group were lower than those in the control group (P < 0.05).
Both the modified closed biopsy technique and needle aspiration biopsy can provide sufficient biopsy tissue for the diagnosis of VX2-transplanted bone tumor in rabbits. At the same time, the improved closed biopsy technique has a certain application value in preventing local metastasis of tumor cells along the puncture channel.
本研究旨在探讨改良闭合活检技术在兔 VX2 移植骨肿瘤模型穿刺活检中的应用价值。
通过胫骨平台将 VX2 肿瘤移植到 30 只兔子的双侧胫骨中，制成 VX2 移植骨肿瘤模型。建模后 7 天，在 X 线引导下进行胫骨近端活检，对活检标本进行病理检查。左腿采用改良闭合活检技术（实验组）活检，右腿采用空心针（对照组）活检。建模后 14 天，所有兔子在 X 线检查后处死，取穿刺孔周围软组织进行病理检查，采用酶联免疫吸附试验（ELISA）检测组织提取物中 PCNA 和 CD34 的表达水平。
实验结束时，共有 3 只兔子死亡，最终纳入研究的兔子共有 27 只。所有穿刺活检获得的髓内标本均检测到肿瘤细胞。建模后 14 天，X 线显示实验组骨膜反应和穿刺孔周围骨外高密度影的发生率为 14.81%（4/27），对照组为 40.74%（11/27），差异有统计学意义（P<0.05）。穿刺孔周围软组织的病理结果显示，实验组肿瘤细胞转移率为 29.63%（8/27），对照组为 100%（27/27），差异有统计学意义（P<0.05）。实验组 PCNA 和 CD34 的表达水平低于对照组（P<0.05）。
改良闭合活检技术和针吸活检均可为兔 VX2 移植骨肿瘤的诊断提供充足的活检组织。同时，改良闭合活检技术在预防肿瘤细胞沿穿刺道局部转移方面具有一定的应用价值。
