Cao Haiying, Jin Yu, Zhao Jingxin, Feng Zhen, Wei Junqiang, Wang Yu, Lin Jing
Chengde Medical College, Chengde, Hebei 067000, P.R. China.
Department of Orthopedics, The Affiliated Hospital of Chengde Medical College, Chengde, Hebei 067000, P.R. China.
Oncol Lett. 2018 Aug;16(2):2299-2304. doi: 10.3892/ol.2018.8881. Epub 2018 Jun 4.
The present study aimed to investigate the efficacy of an improved biopsy method for reducing local exudation and metastasis. After resecting the VX2 tumor in cancer-bearing VX2 rabbits, a tumor cell suspension was prepared. Tumor cells were transplanted into the tibias of 20 rabbits in order to establish a bone tumor model. These adult rabbits underwent a puncture procedure after 2 weeks. All the left tibias were punctured using the improved biopsy method (the experimental group), where bolt closures were implanted into puncture holes through the metallic casing following biopsy. All the right tibias were punctured using a traditional biopsy technique (the control group). Color Doppler ultrasonography was used to assess the exudation of each puncture hole after 1 h. After 7 days, a pathological study was performed on the soft tissue near each puncture hole. The success rate of the bone model was 100% for the 20 rabbits. The exudation rate in the experimental group was lower than that in the control group (25 vs. 80%, respectively; χ=12.13; P<0.05). The tumor-spreading rate in the experimental group was lower than that in the control group (35 vs. 85%, respectively; χ=10.41; P<0.05). Overall, it was shown that the improved biopsy method can prevent local bleeding and tumor metastasis.
本研究旨在探讨一种改进的活检方法在减少局部渗出和转移方面的疗效。在切除荷VX2肿瘤的VX2兔的肿瘤后，制备肿瘤细胞悬液。将肿瘤细胞移植到20只兔的胫骨中以建立骨肿瘤模型。2周后，这些成年兔接受穿刺操作。所有左侧胫骨均采用改进的活检方法进行穿刺（实验组），活检后通过金属套管将螺栓封闭物植入穿刺孔。所有右侧胫骨均采用传统活检技术进行穿刺（对照组）。1小时后，使用彩色多普勒超声评估每个穿刺孔的渗出情况。7天后，对每个穿刺孔附近的软组织进行病理研究。20只兔的骨模型成功率为100%。实验组的渗出率低于对照组（分别为25%和80%；χ=12.13；P<0.05）。实验组的肿瘤扩散率低于对照组（分别为35%和85%；χ=10.41；P<0.05）。总体而言，结果表明改进的活检方法可预防局部出血和肿瘤转移。
