Biological Processes Laboratory (LPB), São Carlos School of Engineering (EESC), University of São Paulo (USP), Av. João Dagnone, 1100, Santa Angelina, 13563-120 São Carlos, São Paulo, Brazil.
Biological Processes Laboratory (LPB), São Carlos School of Engineering (EESC), University of São Paulo (USP), Av. João Dagnone, 1100, Santa Angelina, 13563-120 São Carlos, São Paulo, Brazil.
Bioresour Technol. 2021 Jun;330:124968. doi: 10.1016/j.biortech.2021.124968. Epub 2021 Mar 12.
The down flow structured bed bioreactor (DFSBR) was applied to treat synthetic acid mine drainage (AMD) to reduce sulfate, increase the pH and precipitate metals in solutions (Co, Cu, Fe, Mn, Ni and Zn) using vinasse as an electron donor for sulfate-reducing bacteria (SRB). DFSBR achieved sulfate removal efficiencies between 55 and 91%, removal of Co and Ni were obtained with efficiencies greater than 80%, while Fe, Zn, Cu and Mn were removed with average efficiencies of 70, 80, 73 and 60%, respectively. Sulfate reduction increased pH from moderately acidic to 6.7-7.5. Modelling data confirmed the experimental results and metal sulfide precipitation was the mainly responsible for metal removal. The main genera responsible for sulfate and metal reduction were Geobacter and Desulfovibrio while fermenters were Parabacteroides and Sulfurovum. Moreover, in syntrophism with SRB, they played an important role in the efficiency of metal and sulfate removal.
下流式结构化床生物反应器（DFSBR）被应用于处理人工酸化矿山排水（AMD），以减少硫酸盐、提高 pH 值，并沉淀溶液中的金属（Co、Cu、Fe、Mn、Ni 和 Zn），使用酒糟作为硫酸盐还原菌（SRB）的电子供体。DFSBR 实现了 55%至 91%的硫酸盐去除效率，Co 和 Ni 的去除效率大于 80%，而 Fe、Zn、Cu 和 Mn 的平均去除效率分别为 70%、80%、73%和 60%。硫酸盐还原作用将 pH 值从中性到 6.7-7.5 提高。模型数据证实了实验结果，并且金属硫化物沉淀是主要负责金属去除的原因。负责硫酸盐和金属还原的主要属是 Geobacter 和 Desulfovibrio，而发酵菌是 Parabacteroides 和 Sulfurovum。此外，在与 SRB 的共生作用中，它们在金属和硫酸盐去除的效率中发挥了重要作用。
