Lobanenkov V V
Mol Biol (Mosk). 1988 Jan-Feb;22(1):77-85.
According to the three-dimensional structure of DNase I and the mechanism of its action on linear double-stranded DNA, helix regions in conformations considerably different from the canonical B-form should be resistant to endonucleolysis. A number of DNA sequences specifically bound by nonhistone factors within 5'-flanking regions of the chicken beta A-globin, beta H-globin and c-myc genes are shown to contain short DNase I-resistant DNA domains. Several examples of the occurrence of such DNase I-resistant domains within the sites for high-specific recognition by different proteins are given. The role of the DNA structural polymorphism in site-specific interaction with protein factors is discussed.
根据脱氧核糖核酸酶I的三维结构及其对线性双链DNA的作用机制，与典型B型构象有显著差异的螺旋区域应能抵抗核酸内切酶的作用。已表明，鸡βA -珠蛋白、βH -珠蛋白和c - myc基因5'侧翼区域内被非组蛋白因子特异性结合的一些DNA序列含有短的脱氧核糖核酸酶I抗性DNA结构域。给出了在不同蛋白质进行高特异性识别的位点内出现这种脱氧核糖核酸酶I抗性结构域的几个例子。讨论了DNA结构多态性在与蛋白质因子的位点特异性相互作用中的作用。
