Department of Dermatology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Department of Pathology, Rush University Medical Center, Chicago, Illinois, USA.
J Cutan Pathol. 2021 Aug;48(8):1027-1033. doi: 10.1111/cup.13987. Epub 2021 Apr 6.
Ocular adnexal (OA) sebaceous carcinoma is an aggressive malignancy. Oncologic drivers of ocular sebaceous carcinoma are incompletely understood.
A retrospective search of our pathology archives for OA sebaceous carcinoma identified 18 primary resection specimens. Immunohistochemistry for p16 and ZEB1 and RNA in situ hybridization for high-risk human papillomavirus (HPV) subtypes were performed.
High-risk HPV was demonstrated in 2/11 (18%) cases. p16 overexpression was observed in 10/11 (91%). No association between gender, age at presentation, tumor location, intraepithelial spread, tumor size, and T stage was observed between HPV-driven and nonviral cases. High expression of ZEB1 was observed in the intraepithelial component of 4/14 (28%) cases and in the subepithelial component of 1/13 (7%) cases. ZEB1 overexpression was not associated with HPV status, T stage, or tumor size.
As previously described by others, our findings suggest that a subset of OA sebaceous carcinomas may arise via an HPV-dependent pathway. However, unlike high-risk HPV-driven carcinomas of the oropharynx, we did not identify an association between HPV-status and prognostic features. Furthermore, p16 expression was not a useful surrogate marker for HPV-driven disease. ZEB1 overexpression is not associated with HPV in our cohort of ocular sebaceous carcinoma.
眼附属器（OA）皮脂腺癌是一种侵袭性恶性肿瘤。眼部皮脂腺癌的肿瘤发生驱动因素尚未完全清楚。
通过对我们的病理档案进行回顾性搜索，确定了 18 例原发性切除标本的 OA 皮脂腺癌。对 p16 和 ZEB1 进行免疫组织化学染色，对高危型人乳头瘤病毒（HPV）亚型进行 RNA 原位杂交。
在 2/11（18%）例中检测到高危 HPV。在 10/11（91%）例中观察到 p16 过表达。HPV 驱动型和非病毒型病例之间，在性别、发病时年龄、肿瘤位置、上皮内扩散、肿瘤大小和 T 分期之间未观察到相关性。在 4/14（28%）例的上皮内成分和 1/13（7%）例的上皮下成分中观察到 ZEB1 高表达。ZEB1 过表达与 HPV 状态、T 分期或肿瘤大小无关。
与其他人之前的描述一致，我们的研究结果表明，一部分 OA 皮脂腺癌可能通过 HPV 依赖性途径发生。然而，与口咽部高危 HPV 驱动型癌不同，我们未发现 HPV 状态与预后特征之间的相关性。此外，p16 表达不能作为 HPV 驱动性疾病的有用替代标志物。在我们的眼附属器皮脂腺癌队列中，ZEB1 过表达与 HPV 无关。
