眼附属器皮脂腺癌中的高危型人乳头瘤病毒和 ZEB1。

High-risk human papillomavirus and ZEB1 in ocular adnexal sebaceous carcinoma.

机构信息

Department of Dermatology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Department of Pathology, Rush University Medical Center, Chicago, Illinois, USA.

出版信息

J Cutan Pathol. 2021 Aug;48(8):1027-1033. doi: 10.1111/cup.13987. Epub 2021 Apr 6.

DOI:10.1111/cup.13987

PMID:33745190

Abstract

BACKGROUND

Ocular adnexal (OA) sebaceous carcinoma is an aggressive malignancy. Oncologic drivers of ocular sebaceous carcinoma are incompletely understood.

METHODS

A retrospective search of our pathology archives for OA sebaceous carcinoma identified 18 primary resection specimens. Immunohistochemistry for p16 and ZEB1 and RNA in situ hybridization for high-risk human papillomavirus (HPV) subtypes were performed.

RESULTS

High-risk HPV was demonstrated in 2/11 (18%) cases. p16 overexpression was observed in 10/11 (91%). No association between gender, age at presentation, tumor location, intraepithelial spread, tumor size, and T stage was observed between HPV-driven and nonviral cases. High expression of ZEB1 was observed in the intraepithelial component of 4/14 (28%) cases and in the subepithelial component of 1/13 (7%) cases. ZEB1 overexpression was not associated with HPV status, T stage, or tumor size.

CONCLUSION

As previously described by others, our findings suggest that a subset of OA sebaceous carcinomas may arise via an HPV-dependent pathway. However, unlike high-risk HPV-driven carcinomas of the oropharynx, we did not identify an association between HPV-status and prognostic features. Furthermore, p16 expression was not a useful surrogate marker for HPV-driven disease. ZEB1 overexpression is not associated with HPV in our cohort of ocular sebaceous carcinoma.

摘要

背景

眼附属器（OA）皮脂腺癌是一种侵袭性恶性肿瘤。眼部皮脂腺癌的肿瘤发生驱动因素尚未完全清楚。

方法

通过对我们的病理档案进行回顾性搜索，确定了 18 例原发性切除标本的 OA 皮脂腺癌。对 p16 和 ZEB1 进行免疫组织化学染色，对高危型人乳头瘤病毒（HPV）亚型进行 RNA 原位杂交。

结果

在 2/11（18%）例中检测到高危 HPV。在 10/11（91%）例中观察到 p16 过表达。HPV 驱动型和非病毒型病例之间，在性别、发病时年龄、肿瘤位置、上皮内扩散、肿瘤大小和 T 分期之间未观察到相关性。在 4/14（28%）例的上皮内成分和 1/13（7%）例的上皮下成分中观察到 ZEB1 高表达。ZEB1 过表达与 HPV 状态、T 分期或肿瘤大小无关。

结论

与其他人之前的描述一致，我们的研究结果表明，一部分 OA 皮脂腺癌可能通过 HPV 依赖性途径发生。然而，与口咽部高危 HPV 驱动型癌不同，我们未发现 HPV 状态与预后特征之间的相关性。此外，p16 表达不能作为 HPV 驱动性疾病的有用替代标志物。在我们的眼附属器皮脂腺癌队列中，ZEB1 过表达与 HPV 无关。