Rotterdam Eye Hospital, Schiedamse Vest 180, 3011 BH Rotterdam, The Netherlands.
Department of Ophthalmology, Erasmus MC University Medical Center Rotterdam, Doctor Molewaterplein 40, 3015 GD Rotterdam, The Netherlands.
Int J Mol Sci. 2022 Apr 28;23(9):4877. doi: 10.3390/ijms23094877.
An Ocular Sebaceous Carcinoma (OSC) is a rare malignant tumor for which initial clinical and pathological diagnosis is often incorrect. OSCs can mimic Squamous Cell Carcinomas of the Conjunctiva (SCCC). The aim of this study was to find microRNA biomarkers to distinguish OSCs and SCCCs from normal tissue and from each other. Clinical OSC and SCCC case files and the corresponding histopathological slides were collected and reviewed. Micro dissected formalin-fixed paraffin-embedded tumor and control tissues were subjected to semi-high throughput microRNA profiling. MicroRNA expression distinguishes OSCs and SCCCs from corresponding control tissues. Selected differentially expressed miRNAs were validated using single RT-PCR assays. No prognostic miRNAs could be identified that reliably predict SCCC metastasis or OSC recurrence. A comparison between OSCs (n = 14) and SCCCs (n = 18) revealed 38 differentially expressed microRNAs (p < 0.05). Differentially expressed miRNAs were selected for validation in the discovery cohort and an independent validation cohort (OSCs, n = 11; SCCCs, n = 12). At least two miRNAs, miR-196b-5p (p ≤ 0.05) and miR-107 (p ≤ 0.001), displayed a statistically significant differential expression between OSCs and SCCCs with miR-196b-5p upregulated in SCCCs and miR-107 upregulated in OSCs. In the validation cohort, microRNA miR-493-3p also showed significant upregulation in SCCCs when compared to OSCs (p ≤ 0.05). ROC analyses indicated that the combined miR-196b-5p and miR-107 expression levels predicted OSCs with 90.0% sensitivity and 83.3% specificity. In conclusion, the combined testing of miR-196b-5p and miR-107, can be of additional use in routine diagnostics to discriminate OSCs from SCCCs.
眼附属器皮脂腺癌（OSC）是一种罕见的恶性肿瘤，其初始临床和病理诊断通常不正确。OSC 可模拟结膜鳞状细胞癌（SCCC）。本研究旨在寻找 microRNA 生物标志物，以区分 OSC 和 SCCC 与正常组织，并区分彼此。收集并回顾了临床 OSC 和 SCCC 病例档案和相应的组织病理学切片。对 micro 解剖的福尔马林固定石蜡包埋肿瘤和对照组织进行半高通量 microRNA 分析。miRNA 表达可区分 OSC 和 SCCC 与相应对照组织。使用单个 RT-PCR 测定验证选定的差异表达 miRNA。未鉴定出可可靠预测 SCCC 转移或 OSC 复发的预后 miRNA。OSC（n=14）和 SCC（n=18）之间的比较显示 38 个差异表达的 microRNA（p<0.05）。选择差异表达的 miRNA 进行发现队列和独立验证队列（OSC，n=11；SCC，n=12）的验证。至少两种 miRNA，miR-196b-5p（p≤0.05）和 miR-107（p≤0.001），在 OSC 和 SCC 之间显示出统计学上显著的差异表达，miR-196b-5p 在 SCC 中上调，miR-107 在 OSC 中上调。在验证队列中，miR-493-3p 与 OSC 相比在 SCC 中也显示出显著上调（p≤0.05）。ROC 分析表明，miR-196b-5p 和 miR-107 的联合表达水平可预测 OSC，敏感性为 90.0%，特异性为 83.3%。总之，miR-196b-5p 和 miR-107 的联合检测可在常规诊断中额外用于区分 OSC 和 SCC。
