Department of Laboratory Medicine, Shengjing Hospital of China Medical University, Shenyang, China.
Department of Laboratory Medicine, The People's Hospital of Liupanshui City, Liupanshui, China.
J Clin Lab Anal. 2021 Apr;35(4):e23737. doi: 10.1002/jcla.23737. Epub 2021 Mar 21.
To find new diagnostic markers for idiopathic membranous nephropathy (IMN) and also conduct preliminary explorations into the possible pathogenesis of IMN by comparing the expression of microRNA-451a (miR-451a), miR-106a, miR-19b, miR-17, and phosphatase and tensin homolog (PTEN) protein in the serum of patients with IMN and healthy controls.
The expression levels of miR-451a, miR-106a, miR-19b, and miR-17 in the serum of patients in the IMN group (n = 55, age: 50.2 ± 12.1 years) and the control group (n = 58, age 47.4 ± 13.1 years) were measured by quantitative real-time polymerase chain reaction (qRT-PCR), and the concentration of serum PTEN protein was determined by enzyme-linked immunosorbent assay (ELISA).
Compared with the control group, the expression of miR-106a, miR-19b, and miR-17 was decreased significantly in the IMN group, whereas PTEN protein concentration was increased significantly in the IMN group. The areas under the receiver operating characteristic curve (AUC) of serum miR-106a, miR-19b, miR-17, and PTEN were 0.66 (95% confidence interval [CI], 0.56-0.76), 0.81 (95% CI, 0.73-0.89), 0.69 (95% CI, 0.59-0.79), and 0.86 (95% CI, 0.79-0.93), respectively. The level of serum PTEN protein was negatively correlated with the expression of miR-106a and miR-19b. PTEN concentration was positively correlated with serum urea (Urea), creatinine (Crea), cystatin C (Cysc), 24 h urine total protein (24 h-UP) and negatively correlated with albumin (Alb) and estimated glomerular filtration rate (eGFR).
MiR-106a, miR-19b, miR-17, and PTEN are involved in the pathogenesis of IMN and may become new biomarkers for the diagnosis of IMN.
通过比较特发性膜性肾病（IMN）患者和健康对照者血清中 microRNA-451a（miR-451a）、miR-106a、miR-19b、miR-17 和磷酸酶和张力蛋白同源物（PTEN）蛋白的表达，寻找新的特发性膜性肾病的诊断标志物，并初步探讨特发性膜性肾病的可能发病机制。
采用实时荧光定量聚合酶链反应（qRT-PCR）检测 55 例 IMN 患者（年龄 50.2±12.1 岁）和 58 例健康对照者（年龄 47.4±13.1 岁）血清中 miR-451a、miR-106a、miR-19b 和 miR-17 的表达水平，酶联免疫吸附试验（ELISA）法检测血清中 PTEN 蛋白浓度。
与对照组相比，IMN 组 miR-106a、miR-19b 和 miR-17 的表达明显降低，而 PTEN 蛋白浓度明显升高。血清 miR-106a、miR-19b、miR-17 和 PTEN 的受试者工作特征曲线（ROC）曲线下面积分别为 0.66（95%置信区间：0.56-0.76）、0.81（95%置信区间：0.73-0.89）、0.69（95%置信区间：0.59-0.79）和 0.86（95%置信区间：0.79-0.93）。血清 PTEN 蛋白水平与 miR-106a 和 miR-19b 的表达呈负相关。PTEN 浓度与血清尿素（Urea）、肌酐（Crea）、胱抑素 C（Cysc）、24 h 尿总蛋白（24 h-UP）呈正相关，与白蛋白（Alb）和估算肾小球滤过率（eGFR）呈负相关。
miR-106a、miR-19b、miR-17 和 PTEN 参与了特发性膜性肾病的发病机制，可能成为特发性膜性肾病诊断的新标志物。
