Zhang Zilong, Cui Feifei, Zhou Murong, Wu Song, Zou Quan, Gao Bo
Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu 610054, China.
College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
Curr Gene Ther. 2021;21(4):338-348. doi: 10.2174/1566523221666210319104752.
Lung adenocarcinoma (LADC) is the most common type of lung cancer and is a subtype of non-small-cell lung cancer (NSCLC). Approximately 40% of LADC patients experience brain metastases (BMs) during the course of the disease. In this study, integrated bioinformatics methods were applied to identify key genes related to brain metastasis in lung adenocarcinoma.
We derived and characterized genes differentially expressed between the primary tumour and brain metastases using tumour cells isolated from two lung cancer Patient-derived xenografts (PDX) cases (GSE 69405). Gene ontology (GO) and KEGG pathway enrichment analyses were applied, and protein-protein interaction (PPI) networks and Cytoscape software were utilized to identify key genes.
Four key genes, including CKAP4 (Cytoskeleton Associated Protein 4), SERPINA1 (Serpin Family A Member 1), SDC2 (Syndecan 2) and GNG11 (G Protein Subunit Gamma 11) were identified for BM-LADC by the Venn diagram.
We believe these key genes may be potential biomarkers for improved prognosis and treatment of lung adenocarcinoma.
肺腺癌（LADC）是肺癌最常见的类型，是非小细胞肺癌（NSCLC）的一种亚型。约40%的肺腺癌患者在病程中会发生脑转移（BMs）。在本研究中，应用综合生物信息学方法来识别与肺腺癌脑转移相关的关键基因。
我们使用从两个肺癌患者来源的异种移植（PDX）病例（GSE 69405）中分离的肿瘤细胞，推导并表征了原发性肿瘤和脑转移之间差异表达的基因。应用基因本体（GO）和KEGG通路富集分析，并利用蛋白质-蛋白质相互作用（PPI）网络和Cytoscape软件来识别关键基因。
通过维恩图为脑转移肺腺癌（BM-LADC）鉴定出四个关键基因，包括细胞骨架相关蛋白4（CKAP4）、丝氨酸蛋白酶抑制剂家族A成员1（SERPINA1）、多配体蛋白聚糖2（SDC2）和G蛋白亚基γ11（GNG11）。
我们认为这些关键基因可能是改善肺腺癌预后和治疗的潜在生物标志物。
