一种快速可靠的杂交瘤筛选方法，适用于生产具有功能结构识别的单克隆抗体。

Rapid and reliable hybridoma screening method that is suitable for production of functional structure-recognizing monoclonal antibody.

机构信息

Laboratory of Molecular Biology, Graduate School of Engineering, Yokohama National University, Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan.

Laboratory of Molecular Biology, College of Engineering Sciences, Yokohama National University, Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan.

出版信息

J Biosci Bioeng. 2021 Jun;131(6):696-702. doi: 10.1016/j.jbiosc.2021.02.006. Epub 2021 Mar 19.

DOI:10.1016/j.jbiosc.2021.02.006

PMID:33745778

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7972868/

Abstract

Monoclonal antibodies are extremely valuable functional biomaterials that are widely used not only in life science research but also in antibody drugs and test drugs. There is also a strong need to develop high-quality neutralizing antibodies as soon as possible in order to stop the rapid spread of new infectious diseases such as the SARS-CoV-2 virus. This study has developed a membrane-type immunoglobulin-directed hybridoma screening (MIHS) method for obtaining high-quality monoclonal antibodies with high efficiency and high speed. In addition to these advantages, this paper demonstrates that the MIHS method can selectively obtain monoclonal antibodies that specifically recognize the functional structure of proteins. The MIHS method is a useful technology that greatly contributes to the research community because it can be easily introduced in any laboratory that uses a flow cytometer.

摘要

单克隆抗体是极其有价值的功能生物材料，不仅广泛应用于生命科学研究，还应用于抗体药物和检测药物。为了阻止 SARS-CoV-2 等新传染病的快速传播，也强烈需要尽快开发高质量的中和抗体。本研究开发了一种膜型免疫球蛋白导向的杂交瘤筛选（MIHS）方法，用于高效、快速地获得高质量的单克隆抗体。除了这些优势，本文还证明了 MIHS 方法可以选择性地获得特异性识别蛋白质功能结构的单克隆抗体。MIHS 方法是一种有用的技术，因为它可以很容易地在任何使用流式细胞仪的实验室中引入，因此极大地为研究界做出了贡献。