一种用于食品和饲料中黄曲霉毒素 B 早期检测的动态和拟均相 MBs-icELISA 方法。

A Dynamic and Pseudo-Homogeneous MBs-icELISA for the Early Detection of Aflatoxin B in Food and Feed.

机构信息

School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China.

Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China.

出版信息

Toxins (Basel). 2023 Nov 16;15(11):660. doi: 10.3390/toxins15110660.

Aflatoxin B (AFB) is one of the most toxic and harmful fungal toxins to humans and animals, and the fundamental way to prevent its entry into humans is to detect its presence in advance. In this paper, the monoclonal antibody mAbA2-2 was obtained via three-step sample amplification and multi-concentration standard detection using a subcloning method based on the limited dilution method with AFB as the target. A dynamic and pseucdo-homogeneous magnetic beads enzyme-linked immunosorbent assay (MBs-icELISA) was established using the prepared antibody as the recognition element and immunomagnetic beads as the antigen carrier. The MBs-icELISA showed good linear correlation in the concentration range of 0.004-10 ng/mL with R = 0.99396. The limit of detection (LOD) of the MBs-icELISA for AFB was 0.0013 ng/mL. This new ELISA strategy significantly shortened AFB detection time through improved sensitivity compared to the conventional ELISA method.

黄曲霉毒素 B（AFB）是对人类和动物最具毒性和危害性的真菌毒素之一，防止其进入人体的根本方法是提前检测其存在。本文采用三步样品放大和多浓度标准检测，基于有限稀释法的亚克隆方法，以 AFB 为靶标，获得了单克隆抗体 mAbA2-2。以制备的抗体为识别元件，免疫磁珠为抗原载体，建立了动态和伪均相磁珠酶联免疫吸附测定（MBs-icELISA）。MBs-icELISA 在 0.004-10ng/mL 的浓度范围内具有良好的线性相关性，R=0.99396。MBs-icELISA 检测 AFB 的检出限（LOD）为 0.0013ng/mL。与传统 ELISA 方法相比，这种新的 ELISA 策略通过提高灵敏度显著缩短了 AFB 的检测时间。