Nayak Ankeeta, Majumdar R K, Mehta Naresh K, Mohanty Upasana, Samantaray Swapnarani
College of Fisheries, Central Agricultural University, Lembucherra, Tripura 799 210 India.
J Food Sci Technol. 2021 Apr;58(4):1585-1592. doi: 10.1007/s13197-020-04671-4. Epub 2020 Aug 8.
In this study, influence of feeding habits of fish on the activity of collagenolytic proteases (CP) has been investigated. CP from the visceral waste of freshwater fish (Pangas, Rohu and Common carp) of different feeding habits was isolated and partially purified by 2-steps, (NH)SO fractionation and dialysis. Enzymatic activity and purification fold was determined in each step. The molecular mass of the enzymes were close to that of serine collagenases. Enzyme was assayed for temperature and pH optima, effect of sodium chloride and inhibitors. Optimum temperature and pH was 40 °C and 7-8 respectively. Soybean trypsin inhibitor inhibited the enzyme activity, whereas, EDTA exerted no effect, led to confirmation of serine collagenases. CP of carnivore was more active over a wide range of temperature and pH compared to herbivore and omnivore. The study revealed that the feeding habit of fish play decides the optimal physiological conditions for maximum activity of CP.
在本研究中，已对鱼类摄食习惯对胶原分解蛋白酶（CP）活性的影响进行了调查。从不同摄食习惯的淡水鱼（苏氏圆腹芒、印度魮和鲤鱼）的内脏废弃物中分离出CP，并通过两步法（硫酸铵分级分离和透析）进行部分纯化。在每一步中测定酶活性和纯化倍数。这些酶的分子量与丝氨酸胶原酶的分子量相近。对酶进行了温度和pH最佳值、氯化钠和抑制剂作用的测定。最佳温度和pH分别为40℃和7 - 8。大豆胰蛋白酶抑制剂抑制酶活性，而乙二胺四乙酸无作用，这证实了该酶为丝氨酸胶原酶。与草食性和杂食性鱼类相比，肉食性鱼类的CP在较宽的温度和pH范围内更具活性。该研究表明，鱼类的摄食习惯决定了CP最大活性的最佳生理条件。
