Elseadawy Rana, Abbas Ibrahim, Al-Araby Moustafa, Abu-Elwafa Salah
Parasitology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516 Egypt.
J Parasit Dis. 2021 Mar;45(1):218-227. doi: 10.1007/s12639-020-01299-1. Epub 2020 Nov 1.
Nematodes of genus can cause remarkable economic losses in the small ruminant`s industry and some species have a zoonotic potential. Since the most common source for human infection is the infected animals, accurate identification of different species in animals would reflect the species that can infect humans from the same area. The objective of the present study is to identify common species infecting small ruminants in Dakahlia governorate, Egypt using molecular-based techniques. Fecal samples from 340 sheep and 115 goats from rural areas in 3 cities of Dakahlia governorate and 2 cities at its borders were collected, and the strongyle-type eggs were detected in 33.2% and 14.7% of sheep and goats, respectively. PCR amplification of the internal transcribed spacer of the ribosomal DNA (ITS2 rDNA) for 3 spp.; , and was conducted for eggs harvested from 25 sheep and 16 goat samples. Two species were detected; (in 16 sheep and 14 goats) and (in 2 sheep but no goats). This is the first report of in sheep from Dakahlia governorate, where this species was reported earlier from humans. No was detected in any tested sample of sheep or goats. Purified PCR products of isolates were successfully sequenced and revealed 3 haplotypes; 2 from sheep and 1 from goats, and the isolates are related genetically to isolates from camels in Egypt. Phylogenetic analysis of the Genbank-retrieved ITS2-amplified isolates worldwide suggested the existence of genetic variants. Earlier reports on the identified spp. in different animals from Egypt as well as African and Arabian countries are tabulated.
属线虫可在小型反刍动物产业中造成显著经济损失，且某些物种具有人畜共患病潜力。由于人类感染的最常见来源是受感染动物，准确鉴定动物体内不同的物种将反映出同一地区可能感染人类的物种。本研究的目的是使用基于分子的技术鉴定埃及达卡利亚省感染小型反刍动物的常见物种。采集了达卡利亚省3个城市农村地区以及其边境2个城市的340只绵羊和115只山羊的粪便样本，绵羊和山羊中分别有33.2%和14.7%检测到圆线虫卵。对从25份绵羊样本和16份山羊样本中收获的虫卵进行了核糖体DNA内部转录间隔区（ITS2 rDNA）的PCR扩增，用于3个物种；、和。检测到两个物种；（16只绵羊和14只山羊中）和（2只绵羊中有，但山羊中没有）。这是达卡利亚省绵羊中首次报告该物种，该物种此前曾在人类中报告过。在任何绵羊或山羊测试样本中均未检测到。分离株的纯化PCR产物成功测序，显示出3种单倍型；2种来自绵羊，1种来自山羊，这些分离株在基因上与埃及骆驼的分离株相关。对从Genbank检索到的全球ITS2扩增分离株的系统发育分析表明存在基因变体。列出了埃及以及非洲和阿拉伯国家不同动物中已鉴定物种的早期报告。
