Javaid Usman, Javaid Sana, Ashraf Waseem, Rasool Muhammad Fawad, Noman Omar M, Alqahtani Ali S, Majeed Abdul, Shakeel Waleed, Albekairi Thamer H, Alqahtani Faleh, Imran Imran
Department of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan.
Department of Pharmacy, The Women University, Multan, Pakistan.
Dose Response. 2021 Mar 4;19(1):1559325821998486. doi: 10.1177/1559325821998486. eCollection 2021 Jan-Mar.
The current study was planned to investigate the pharmacological basis of extract (SV.CR) pertaining to anxiolytic, antidepressant and memory-enhancing effects in rats. The SV.CR was analyzed in-vitro for phytoconstituents, antioxidant potential and anticholinesterase activity. The rats treated in a dose-dependent manner (25, 50 and 100 mg/kg of SV.CR) were subjected to behavioral tests for anxiety, depression and memory judgment followed by biochemical studies. A notable dose-dependent anxiolytic potential of SV.CR was observed in elevated plus maze and open field tests (P < 0.05). The decreased immobility time of the treated rats in the forced swim test (P < 0.01) unveiled the plant's potential to reduce depression. Moreover, SV.CR treatment also reversed scopolamine-impaired cognition (P < 0.05) in various deployed memory and learning tasks. Biochemical studies of brain homogenates of SV.CR treated animals demonstrated decreased anticholinesterase activity and lipid peroxidation levels whereas increased levels of superoxide dismutase and glutathione peroxidase (P < 0.05 vs scopolamine group) were noted. The scientific validation of the study supported the use of in reducing anxiety, depression and amnesia in experimental models. Phytoconstituents in SV.CR such as oleanolic acid and caffeic acid might have played a significant neuroprotective role via modulation of oxidative stress and neurochemical aspects.
本研究旨在探究提取物（SV.CR）对大鼠抗焦虑、抗抑郁及增强记忆作用的药理学基础。对SV.CR进行了体外植物成分分析、抗氧化潜力及抗胆碱酯酶活性分析。以剂量依赖方式（25、50和100 mg/kg的SV.CR）处理大鼠后，进行焦虑、抑郁和记忆判断的行为测试，随后进行生化研究。在高架十字迷宫和旷场试验中观察到SV.CR具有显著的剂量依赖性抗焦虑潜力（P < 0.05）。在强迫游泳试验中，处理组大鼠不动时间减少（P < 0.01），揭示了该植物减轻抑郁的潜力。此外，在各种记忆和学习任务中，SV.CR处理还逆转了东莨菪碱损害的认知（P < 0.05）。对SV.CR处理动物脑匀浆的生化研究表明，抗胆碱酯酶活性和脂质过氧化水平降低，而超氧化物歧化酶和谷胱甘肽过氧化物酶水平升高（与东莨菪碱组相比，P < 0.05）。该研究的科学验证支持了SV.CR在实验模型中用于减轻焦虑、抑郁和失忆。SV.CR中的植物成分如齐墩果酸和咖啡酸可能通过调节氧化应激和神经化学方面发挥了重要的神经保护作用。
