Sirikhet Jutiporn, Chanmahasathien Wisinee, Raiwa Araya, Kiattisin Kanokwan
Department of Pharmaceutical Sciences Faculty of Pharmacy Chiang Mai University Chiang Mai Thailand.
Nutraceuticals, and Cosmeceuticals Faculty of Pharmacy Innovation Center for Holistic Heath Chiang Mai University Chiang Mai Thailand.
Food Sci Nutr. 2021 Jan 31;9(3):1750-1760. doi: 10.1002/fsn3.2156. eCollection 2021 Mar.
Lycopene is one of naturally occurring carotenoids in plants including watermelon . Heat, light, and oxygen effect on lycopene isomerization and degradation. Nanostructured lipid carriers (NLCs) are drug delivery system which can enhance the stability of active compound. Therefore, this study aimed to develop watermelon extract loaded in NLCs for lycopene stability improvement. The NLCs were prepared using a hot homogenization technique. Cocoa butter was used as solid lipid. Grape seed oil was used as liquid lipid. Span 80 and Plantasens HE20 were used as an emulsifier. The selected unloaded NLCs contained solid lipid to liquid lipid at the ratio of 3:1 and 10% (w/w) of total lipid. The particle size of watermelon extract-loaded NLCs (WH-loaded NLCs) was 130.17 ± 0.72 nm with low PDI and high zeta potential. It also presented high entrapment efficiency. For stability study, the WH-NLC3 could enhance stability and maintain lycopene content after stability test. It exhibited the highest values of lycopene content (83.26 ± 2.30%) when stored at 4°C. It also possessed a prolonged release pattern over 48 hr. Therefore, the NLCs could improve stability and release profile of lycopene from watermelon extract.
番茄红素是包括西瓜在内的植物中天然存在的类胡萝卜素之一。热、光和氧气会影响番茄红素的异构化和降解。纳米结构脂质载体（NLCs）是一种药物递送系统，可提高活性化合物的稳定性。因此，本研究旨在开发负载于NLCs中的西瓜提取物，以提高番茄红素的稳定性。采用热均质技术制备NLCs。可可脂用作固体脂质。葡萄籽油用作液体脂质。司盘80和植物源乳化剂HE20用作乳化剂。选定的未负载NLCs中固体脂质与液体脂质的比例为3:1，占总脂质的10%（w/w）。负载西瓜提取物的NLCs（WH-NLCs）的粒径为130.17±0.72nm，具有低多分散指数和高zeta电位。其包封率也很高。对于稳定性研究，WH-NLC3在稳定性测试后可提高稳定性并保持番茄红素含量。在4°C储存时，其番茄红素含量最高（83.26±2.30%）。它还具有超过48小时的缓释模式。因此，NLCs可以提高西瓜提取物中番茄红素的稳定性和释放特性。
