Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian 116600, China.
School of Materials Science and Engineering, Zhengzhou University, Zhengzhou 450001, China.
ACS Nano. 2021 Apr 27;15(4):6517-6529. doi: 10.1021/acsnano.0c08790. Epub 2021 Mar 22.
The combination of photothermal therapy (PTT) and gene therapy (GT) shows great potential to achieve synergistic anti-tumor activity. However, the lack of a controlled release of genes from carriers remains a severe hindrance. Herein, peptide lipid (PL) and sucrose laurate (SL) were used to coat single-walled carbon nanotubes (SCNTs) and multi-walled carbon nanotubes (MCNTs) to form bifunctional delivery systems (denoted SCNT-PS and MCNT-PS, respectively) with excellent temperature-sensitivity and photothermal performance. CNT/siRNA suppressed tumor growth by silencing survivin expression while exhibiting photothermal effects under near-infrared (NIR) light. SCNT-PS/siRNA showed very high anti-tumor activity, resulting in the complete inhibition of some tumors. It was highly efficient for systemic delivery to tumor sites and to facilitate siRNA release owing to the phase transition of the temperature-sensitive lipids, due to PL and SL coating. Thus, SCNT-PS/siRNA is a promising anti-tumor nanocarrier for combined PTT and GT.
光热疗法（PTT）和基因疗法（GT）的联合显示出了实现协同抗肿瘤活性的巨大潜力。然而，载体中基因的控制释放仍然是一个严重的障碍。在此，采用肽脂质（PL）和月桂酰蔗糖（SL）对单壁碳纳米管（SCNT）和多壁碳纳米管（MCNT）进行包覆，形成具有优异温度敏感性和光热性能的双功能递药系统（分别表示为 SCNT-PS 和 MCNT-PS）。CNT/siRNA 通过沉默生存素的表达来抑制肿瘤生长，同时在近红外（NIR）光下表现出光热效应。SCNT-PS/siRNA 表现出非常高的抗肿瘤活性，导致一些肿瘤完全被抑制。由于 PL 和 SL 的包覆，由于温度敏感脂质的相变，SCNT-PS/siRNA 对肿瘤部位的全身递送非常高效，并有助于 siRNA 的释放。因此，SCNT-PS/siRNA 是一种很有前途的用于联合 PTT 和 GT 的抗肿瘤纳米载体。
