Park Arum, Yang Yunjeong, Jo Junyoung, Yoon Suk Ran
Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
Department of Biochemistry, College of pharmacy, Chungnam National University, Daejeon, Korea.
Andrologia. 2021 Jun;53(5):e14025. doi: 10.1111/and.14025. Epub 2021 Mar 22.
Korean herbal formulation, MYOMI-14, has been reported to improve the idiopathic male infertility condition with poor semen. In this study, four MYOMI formulations were modified from MYOMI-14 by reducing the number of constituents. We investigated the therapeutic effect of MYOMI formulations on cyclophosphamide-induced male infertility using mice model. Cyclophosphamide treatment significantly decreased body weight, testicular weight, sperm count, normal-shaped sperm rate and sperm motility compared to untreated control group, whereas MYOMI formulations restore the cyclophosphamide-induced dysfunction, as determined by increased sperm count and motility, and decreased abnormally shaped spermatozoa. In addition, treatment with MYOMI formulations reduced cyclophosphamide-induced apoptosis of germ cells and oxidative stress. MYOMI-treated mice also showed improved spermatogenesis as shown by the increased expression of spermatogenesis-related genes, as cAMP-responsive element modulator (CREM) and cAMP response element-binding (CREB) protein. Among the MYOMI formulations, MYOMI-7 showed better results in terms of recovering CP-induced damages to testis and improving the fertility. Taken together, this study is expected to make significant contribution to the literature by exploring various formulations that reduced constituents of MYOMI-14, a Korean herbal medicine, in treating CP-induced male infertility.
据报道，韩国草药配方MYOMI - 14可改善精液质量差的特发性男性不育状况。在本研究中，通过减少成分数量，从MYOMI - 14改良了四种MYOMI配方。我们使用小鼠模型研究了MYOMI配方对环磷酰胺诱导的男性不育的治疗效果。与未处理的对照组相比，环磷酰胺处理显著降低了体重、睾丸重量、精子数量、正常形态精子率和精子活力，而MYOMI配方可恢复环磷酰胺诱导的功能障碍，这通过精子数量和活力增加以及异常形态精子减少来确定。此外，MYOMI配方治疗减少了环磷酰胺诱导的生殖细胞凋亡和氧化应激。经MYOMI处理的小鼠还表现出精子发生改善，如精子发生相关基因（如cAMP反应元件调节剂（CREM）和cAMP反应元件结合（CREB）蛋白）的表达增加所示。在MYOMI配方中，MYOMI - 7在恢复环磷酰胺诱导的睾丸损伤和提高生育能力方面表现出更好的效果。综上所述，本研究有望通过探索减少韩国草药MYOMI - 14成分的各种配方在治疗环磷酰胺诱导的男性不育方面为文献做出重大贡献。
