口腔黏膜下纤维化进展和恶变过程中Th17/Treg平衡的倾斜
Skewed Th17/Treg balance during progression and malignant transformation of oral submucous fibrosis.
作者信息
Liu Sixuan, Liu Ziyi, Shan Zhongyan, Liu Yuxin, Chen Tianjun, Fang Liangjuan, Quan Hongzhi
机构信息
Hunan Key Laboratory of Oral Health Research & Hunan 3D Printing Engineering Research Center of Oral Care & Hunan Clinical Research Center of Oral Major Diseases and Oral Health Xiangya Stomatological Hospital & Xiangya School of Stomatology, Central South University, Changsha, China.
Department of Oral Maxillofacial Surgery, Xiangya Stomatological Hospital & School of Stomatology, Central South University, Changsha, China.
出版信息
Oral Dis. 2022 Nov;28(8):2119-2130. doi: 10.1111/odi.13853. Epub 2021 Aug 17.
OBJECTIVES
The aim of our study was to determine the impact of Th17/Treg imbalance on the progression and malignant transformation of oral submucosal fibrosis (OSF).
MATERIALS AND METHODS
To assess Th17 and Treg expression, overall 52 peripheral blood samples from OSF, oral squamous cell carcinoma (OSCC) patients, and healthy donors were analyzed by flow cytometry. Thirty normal oral mucosa, 72 OSF, and 90 OSCC samples were analyzed by immunohistochemistry.
RESULTS
In peripheral blood samples, in OSCC with OSF, Th17 and Treg expression were significantly higher than those in OSF and OSCC without OSF as confirmed by immunohistochemistry. During OSF progression, Th17 and Th17/Treg ratio showed an increasing trend, while Treg expression showed a decreasing trend. Treg expression was significantly higher in OSCC with OSF than in OSF and OSCC without OSF, whereas the Th17/Treg ratio was significantly lower in OSCC with OSF. Treg expression was significantly correlated with smoking and clinical stage. Th17/Treg ratio was significantly associated with tumor size, lymph node metastasis, and clinical stage. A low Th17/Treg ratio was significantly associated with poor prognosis.
CONCLUSIONS
Th17/Treg ratio is a potential diagnostic indicator for OSF occurrence and malignant transformation and was an independent prognostic factor for OSCC.
目的
本研究旨在确定Th17/Treg失衡对口腔黏膜下纤维化（OSF）进展及恶变的影响。
材料与方法
为评估Th17和Treg表达，采用流式细胞术分析了来自OSF患者、口腔鳞状细胞癌（OSCC）患者及健康供者的共52份外周血样本。采用免疫组织化学方法分析了30份正常口腔黏膜、72份OSF及90份OSCC样本。
结果
在外周血样本中，免疫组织化学证实，合并OSF的OSCC中Th17和Treg表达显著高于未合并OSF的OSF及OSCC。在OSF进展过程中，Th17及Th17/Treg比值呈上升趋势，而Treg表达呈下降趋势。合并OSF的OSCC中Treg表达显著高于未合并OSF的OSF及OSCC，而合并OSF的OSCC中Th17/Treg比值显著更低。Treg表达与吸烟及临床分期显著相关。Th17/Treg比值与肿瘤大小、淋巴结转移及临床分期显著相关。低Th17/Treg比值与预后不良显著相关。
结论
Th17/Treg比值是OSF发生及恶变的潜在诊断指标，也是OSCC的独立预后因素。
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