Laboratorio de Metabolismo de la Diabetes Mellitus, Unidad de Morfofisiología, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, México, Mexico.
Laboratorio de Fitoquímica, Unidad de Biología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, México, Mexico.
Birth Defects Res. 2021 Jul 15;113(12):981-994. doi: 10.1002/bdr2.1894. Epub 2021 Mar 22.
The deleterious effects of diabetes mellitus (DM) over development are apparently due to an increase in oxidative stress. Some antioxidants could prevent developmental alterations produced by diabetic state. Extracts of plants of the genus Buddleja are used traditionally for Mexican indigens to ameliorate some diseases. The purpose of this work was to evaluate the effect of the extract of Buddleja cordata over diabetic embryopathy.
Two experimental approaches were used: an in vivo study and an in vitro model. In the first, rats were treated with streptozotocin, streptozotocin plus methanolic extract of B. cordata, or none. Females were sacrificed at gestational day (GD) 19, and biochemical clinical parameters were measured; also, the fetuses were obtained and morphologically analyzed. In the in vitro model, a verbascoside-enriched fraction (VEF) of the extract was used in whole embryo culture in order to search for the mechanisms for embryoprotection effect over hyperglycemia-induced malformations.
In the in vivo experiments, B. cordata extract reduces the frequency and severity of fetal malformations produced by chemically induced diabetes, and additionally partially ameliorates the diabetic condition; in the in vitro model, both severity and frequency of embryo dysmorphogenesis were reduced by the VEF; also, this fraction reduces lipoperoxidation without affecting the activity of the antioxidant enzymes.
The results suggest that verbascoside of methanolic extract and enriched fraction can directly affect the redox state, and thus, prevents the embryotoxicity mediated by oxidative stress, in embryos of diabetic pregnancy.
糖尿病（DM）对发育的有害影响显然是由于氧化应激增加所致。一些抗氧化剂可以预防糖尿病状态引起的发育改变。醉鱼草属植物的提取物被传统上用于改善一些墨西哥土着人的疾病。这项工作的目的是评估醉鱼草提取物对糖尿病胚胎病的影响。
采用体内和体外两种实验方法。在第一个实验中，大鼠用链脲佐菌素、链脲佐菌素加醉鱼草甲醇提取物或不加处理。在妊娠第 19 天处死雌性，测量生化临床参数；还获得了胎儿并进行了形态学分析。在体外模型中，使用提取物的一种毛蕊花糖苷富集部分（VEF）进行全胚胎培养，以寻找对高血糖诱导的畸形具有胚胎保护作用的机制。
在体内实验中，醉鱼草提取物可降低化学诱导的糖尿病引起的胎儿畸形的频率和严重程度，并且还部分改善了糖尿病状态；在体外模型中，VEF 降低了胚胎发育畸形的严重程度和频率；此外，该部分还降低了脂质过氧化，而不影响抗氧化酶的活性。
这些结果表明，甲醇提取物和富集部分的毛蕊花糖苷可以直接影响氧化还原状态，从而防止糖尿病妊娠中由氧化应激介导的胚胎毒性。
