Khafaga Asmaa F, Elewa Yaser H A, Atta Mustafa S, Noreldin Ahmed E
Pathology Department, Faculty of Veterinary Medicine, Alexandria University, Edfina22758, Egypt.
Histology and Cytology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig44519, Egypt.
Microsc Microanal. 2021 Mar 10:1-13. doi: 10.1017/S1431927621000210.
Renal aging is a progressive, physiological, and anatomical change that naturally occurs in all animal species. To date, no information is available concerning the aging-related structural and functional changes in camel kidneys. A total of 25 healthy male camels (14 aged 4–6 years and 11 aged 18–22 years) were included in this study. After the camels were slaughtered, samples were collected from all the camels’ kidneys and prepared for histopathological, immunohistochemical, and gene expression evaluations. The most striking observation was the significant decline in the immunohistochemical abundance of podocin and the significant upregulation of smoothening in the aging camels’ kidneys. However, the nonsignificant changes have reported for nephrin, calbindin, autophagy 5 (ATG5), aquaporin 1, and toll-like receptor 9. Furthermore, the mRNA expressions of sirtuin 1, superoxide dismutase 1, superoxide dismutase 2, peroxisome proliferator-activated receptor alpha, B-cell lymphoma 2 (Bcl-2), and erythropoietin were significantly decreased in the aging camels’ kidneys. While the significant upregulation of Bcl-2-associated X protein and the nonsignificant increase in ATG5 expression levels were reported in the aging camels’ kidneys. The present findings provide better understanding of the complex events and initiating factors of aging, allowing for the development of a future therapeutic strategy to preserve adequate renal function throughout life.
肾脏老化是一种在所有动物物种中自然发生的渐进性生理和解剖学变化。迄今为止，尚无关于骆驼肾脏与衰老相关的结构和功能变化的信息。本研究共纳入25头健康雄性骆驼（14头年龄在4至6岁，11头年龄在18至22岁）。骆驼被屠宰后，从所有骆驼的肾脏采集样本，准备进行组织病理学、免疫组织化学和基因表达评估。最显著的观察结果是，衰老骆驼肾脏中足突蛋白的免疫组织化学丰度显著下降，而平滑蛋白显著上调。然而，肾素、钙结合蛋白、自噬5（ATG5）、水通道蛋白1和Toll样受体9的变化不显著。此外，衰老骆驼肾脏中沉默调节蛋白1、超氧化物歧化酶1、超氧化物歧化酶2、过氧化物酶体增殖物激活受体α、B细胞淋巴瘤2（Bcl-2）和促红细胞生成素的mRNA表达显著降低。而衰老骆驼肾脏中Bcl-2相关X蛋白显著上调，ATG5表达水平无显著增加。本研究结果有助于更好地理解衰老的复杂过程和起始因素，为制定未来在整个生命过程中维持肾脏功能的治疗策略提供依据。
