Vittorazzi Stenio Eder, Lourenço Luciana Bolsoni, Zattera Michelle Louise, Weber Luiz Norberto, Recco-Pimentel Shirlei Maria, Bruschi Daniel Pacheco
Universidade do Estado de Mato Grosso, Faculdade de Ciências Biológicas, Agrárias e da Saúde, Departamento de Ciências Biológicas, Tangará da Serra, MT, Brazil.
Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Genética, Programa de Pós-Graduação em Genética, Curitiba, PR, Brazil.
Genet Mol Biol. 2021 Mar 22;44(2):e20200301. doi: 10.1590/1678-4685-GMB-2020-0301. eCollection 2021.
The nominal anuran species Crossodactylus gaudichaudii Duméril and Bibron, 1841 and Crossodactylus aeneus Müller, 1924 are indistinguishable based on adult and larval morphology, being subject of taxonomic doubts. Here, we describe the karyotypes of C. gaudichaudii and C. aeneus, using classical and molecular cytogenetic markers. In addition, we used sequences of the H1 mitochondrial DNA to infer their phylogenetic relationships by Maximum Likelihood (ML) and Maximum Parsimony (MP) approaches and species delimitation test (by bPTP approach). The karyotypic data do not differentiate C. gaudichaudii and C. aeneus in any of the chromosome markers assessed. In both phylogenetic analyses, C. gaudichaudii and C. aeneus were recovered into a strongly supported clade. The species delimitation analysis recovered the specimens assigned to C. gaudichaudii and C. aeneus as a single taxonomic unit. Taken the cytogenetic and genetic results together with previous studies of internal and external morphology of tadpoles and biacoustic pattern, C. gaudichaudii and C. aeneus could not be differentiated, which supports the hypothesis that they correspond to the same taxonomic unit, with C. aeneus being a junior synonym of C. gaudichaudii.
1841年由杜梅里尔和比布龙命名的无尾目物种高迪氏多指蛙（Crossodactylus gaudichaudii）以及1924年由米勒命名的铜色多指蛙（Crossodactylus aeneus），基于成体和幼体形态无法区分，存在分类学疑问。在此，我们使用经典和分子细胞遗传学标记描述了高迪氏多指蛙和铜色多指蛙的核型。此外，我们使用线粒体DNA的H1序列，通过最大似然法（ML）和最大简约法（MP）以及物种界定测试（通过bPTP方法）来推断它们的系统发育关系。在评估的任何染色体标记中，核型数据都无法区分高迪氏多指蛙和铜色多指蛙。在两种系统发育分析中，高迪氏多指蛙和铜色多指蛙都被归入一个得到有力支持的分支。物种界定分析将归入高迪氏多指蛙和铜色多指蛙的标本作为一个单一分类单元。综合细胞遗传学和遗传学结果以及之前对蝌蚪内外形态和双声模式的研究，高迪氏多指蛙和铜色多指蛙无法区分，这支持了它们对应于同一分类单元的假设，即铜色多指蛙是高迪氏多指蛙的次异名。
