Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.
Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61-704 Poznan, Poland.
Plant Cell. 2021 Jul 19;33(6):1863-1887. doi: 10.1093/plcell/koab073.
Plants recognize surrounding microbes by sensing microbe-associated molecular patterns (MAMPs) to activate pattern-triggered immunity (PTI). Despite their significance for microbial control, the evolution of PTI responses remains largely uncharacterized. Here, by employing comparative transcriptomics of six Arabidopsis thaliana accessions and three additional Brassicaceae species to investigate PTI responses, we identified a set of genes that commonly respond to the MAMP flg22 and genes that exhibit species-specific expression signatures. Variation in flg22-triggered transcriptome responses across Brassicaceae species was incongruent with their phylogeny, while expression changes were strongly conserved within A. thaliana. We found the enrichment of WRKY transcription factor binding sites in the 5'-regulatory regions of conserved and species-specific responsive genes, linking the emergence of WRKY-binding sites with the evolution of gene expression patterns during PTI. Our findings advance our understanding of the evolution of the transcriptome during biotic stress.
植物通过感知微生物相关分子模式（MAMPs）来识别周围的微生物，从而激活模式触发免疫（PTI）。尽管它们在微生物控制方面具有重要意义，但 PTI 反应的进化在很大程度上仍未得到充分描述。在这里，我们通过对六个拟南芥品系和三个其他十字花科物种进行比较转录组学研究，以调查 PTI 反应，鉴定了一组共同响应 MAMP flg22 的基因和表现出物种特异性表达特征的基因。在十字花科物种中，flg22 触发的转录组反应的变化与它们的系统发育不一致，而在拟南芥中，表达变化则强烈保守。我们发现，保守和特异响应基因的 5'-调控区富集了 WRKY 转录因子结合位点，将 WRKY 结合位点的出现与 PTI 过程中基因表达模式的进化联系起来。我们的研究结果增进了我们对生物胁迫过程中转录组进化的理解。
