生物核磁共振波谱学中的组合多频带去耦。
Combined multi-band decoupling in biomolecular NMR spectroscopy.
机构信息
Bruker BioSpin Corp, Billerica, MA, USA.
Structural Biophysics Laboratory, National Cancer Institute, Frederick, MD, USA.
出版信息
J Biomol NMR. 2021 Mar;75(2-3):89-95. doi: 10.1007/s10858-021-00360-2. Epub 2021 Mar 22.
Abstract
Multi-resonance NMR experiments are powerful analytical and structural tools. Their conceptualization assumes that RF fields may be combined independently to manipulate spin interactions. However, practical implementation can compromise performance. One limitation is the generation of combination bands when two or more RF fields are applied simultaneously within the NMR probe. The combination bands can lead to significant interference with the detection circuitry. A facile approach to combined multi-band decoupling can resolve these problems and increase sensitivity two-fold (or more), by time sharing the application of the individual frequencies rather than time sharing decoupling and data acquisition.
摘要
多共振 NMR 实验是强大的分析和结构工具。它们的概念化假设射频场可以独立组合以操纵自旋相互作用。然而，实际实现可能会影响性能。一个限制是当两个或更多射频场同时应用于 NMR 探头内时会产生组合频带。组合频带会导致与检测电路的严重干扰。通过分时应用各个频率而不是分时应用去耦和数据采集，可以解决这些问题并将灵敏度提高两倍（或更多），这是一种简便的组合多频带去耦方法。
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