Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
Vet Clin Pathol. 2021 Mar;50(1):37-44. doi: 10.1111/vcp.12962. Epub 2021 Mar 22.
Macrophage-like (ML) cells are rarely observed on blood smear examinations, and the significance of these cells has been poorly described.
The objective of this study was to retrospectively describe selected hematologic and clinical characteristics of dogs with ML cells in peripheral blood.
Complete blood count (CBC) reports with blood smear evaluations from the clinical pathology laboratory records at North Carolina University College of Veterinary Medicine were retrospectively reviewed. Data were collected over a 10-year-period. Dogs were defined as having circulating ML cells if three or more ML cells were present on a single blood smear. Hematologic and clinical data of dogs with circulating ML cells were compared with age-matched hospital-derived control dogs.
Of 61,631 CBC records, 87 reports (0.14%) described the presence of ML cells. Thirty-nine dogs met the inclusion criteria. The hemogram of dogs with circulating ML cells was characterized by a pronounced inflammatory and stress leukogram, anemia, and thrombocytopenia. Of the 39 dogs, 19 (49%) had systemic or severe localized inflammatory/necrotic diseases. Eighteen (46%) dogs were diagnosed with neoplasia of histiocytic (5) and non-histiocytic origins (13). Dogs with circulating ML cells had a shorter median survival time (34 days) than the control dogs (595 days, P < .0001), with an increased occurrence of death/euthanasia within 1 month (3.89-fold). However, the presence of circulating ML cells was not found to be an independent prognostic factor in a multivariable model.
The hemograms and diagnoses of dogs with ML cells suggest that severe inflammatory conditions or histiocytic and non-histiocytic neoplasia are common causes for circulating ML cells.
在外周血涂片检查中很少观察到类巨噬细胞（ML）细胞，这些细胞的意义尚未得到充分描述。
本研究旨在回顾性描述外周血中存在 ML 细胞的犬的选定血液学和临床特征。
回顾性分析北卡罗来纳大学兽医学院临床病理实验室记录的全血细胞计数（CBC）报告和血涂片评估。数据收集时间为 10 年。如果在单个血涂片上存在三个或更多 ML 细胞，则将狗定义为存在循环 ML 细胞。将具有循环 ML 细胞的犬的血液学和临床数据与年龄匹配的医院来源的对照犬进行比较。
在 61631 份 CBC 记录中，有 87 份报告（0.14%）描述了 ML 细胞的存在。符合纳入标准的犬有 39 只。具有循环 ML 细胞的犬的血液图像特征为明显的炎症和应激性白细胞增多、贫血和血小板减少。在 39 只犬中，有 19 只（49%）患有全身性或严重局部炎症/坏死性疾病。18 只（46%）犬被诊断为组织细胞起源（5 只）和非组织细胞起源（13 只）的肿瘤。具有循环 ML 细胞的犬的中位存活时间（34 天）短于对照组犬（595 天，P < 0.0001），在 1 个月内死亡/安乐死的发生率增加（3.89 倍）。然而，在多变量模型中，循环 ML 细胞的存在并未被发现是一个独立的预后因素。
具有 ML 细胞的犬的血液图像和诊断提示严重的炎症状态或组织细胞和非组织细胞肿瘤是循环 ML 细胞的常见原因。
