National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Department of Preventive Dentistry, National Clinical Research Center for Oral Diseases, Peking University School and Hospital of Stomatology, Beijing, China.
Joint International Research Center of Translational and Clinical Research, Peking University Health Science Center and King's College London, Beijing & London, China & United Kingdom.
Oral Dis. 2022 Sep;28(6):1640-1651. doi: 10.1111/odi.13838. Epub 2021 Mar 19.
To explore the changes of peptidome profiles of saliva, serum, and gingival crevicular fluid (GCF) before and after non-surgical periodontal treatment in patients with generalized periodontitis (stage I/II).
Saliva, serum, and GCF samples were collected from 17 patients at baseline (T ), one week after ultrasonic supragingival scaling (T ) and eight weeks after subgingival scaling and root planning (T ). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was carried out to detect changes in peptidomic profiles. Then, nano-liquid chromatography-electrospray ionization-tandem mass spectrometry (nano-LC/ESI-MS/MS) was performed to identify potential peptide biomarkers.
Most of the peptides from the patients exhibited a decreasing trend from the time point of pretreatment to that of post-treatment. Cluster analysis and scatter plots using these peptides indicated that salivary peptidome has an acceptable capability of reflecting the status of stage I/II generalized periodontitis. Seven of these peptides were successfully identified as α-1-antitrypsin, immunoglobulin κ variable 4-1, haptoglobin, and immunoglobulin heavy constant γ2.
Certain peptides in saliva, serum, and GCF were down-regulated after non-surgical periodontal treatment, demonstrating the application prospects of saliva in monitoring and surveillance of periodontal diseases in both clinical settings and communities.
探讨广泛性牙周炎（I/II 期）患者牙周非手术治疗前后唾液、血清和龈沟液（gingival crevicular fluid，GCF）中肽谱的变化。
本研究共纳入 17 例患者，分别在基线（T ）、超声龈上洁治后 1 周（T ）和龈下刮治及根面平整后 8 周（T ）时采集唾液、血清和 GCF 样本。采用基质辅助激光解吸电离飞行时间质谱（matrix-assisted laser desorption/ionization time-of-flight mass spectrometry，MALDI-TOF MS）检测肽谱的变化，然后采用纳升液相色谱-电喷雾串联质谱（nano-liquid chromatography-electrospray ionization-tandem mass spectrometry，nano-LC/ESI-MS/MS）鉴定潜在的肽类生物标志物。
大多数肽类物质在治疗前到治疗后的时间点呈下降趋势。使用这些肽类物质进行聚类分析和散点图分析表明，唾液肽谱具有较好的反映 I/II 期广泛性牙周炎状态的能力。其中 7 种肽类物质被成功鉴定为α-1-抗胰蛋白酶、免疫球蛋白κ轻链可变区 4-1、触珠蛋白和免疫球蛋白重链 γ2。
牙周非手术治疗后，唾液、血清和 GCF 中的某些肽类物质下调，表明唾液在牙周病的临床和社区监测中具有应用前景。
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