Marchi Laura Teodoro de, Pirondi Amanda Borges, Ventura Talita Mendes Oliveira, Grizzo Larissa Tercilia, Buzalaf Marília Afonso Rabelo, Foratori-Junior Gerson Aparecido
Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, São Paulo, Brasil.
J Appl Oral Sci. 2025 May 12;33:e20250031. doi: 10.1590/1678-7757-2025-0031. eCollection 2025.
This cross-sectional study investigated the salivary proteomic profile associated with generalized gingivitis in pregnant women with obesity.
Pregnant women in the third trimester (≥27 weeks of gestation) were divided into two groups based on bleeding on probing (BOP): G1 (BOP>50%; n=9) and G2 (BOP 0-30%; n=9). Collected unstimulated saliva samples were individually analyzed using nano liquid chromatography electron spray ionization tandem mass spectrometry. Identified proteins were classified according to gene ontology for biological processes, molecular functions, immune system involvement, and cellular components. Differential protein expression was determined using thresholds of p<0.05 for downregulation and 1-p>0.95 for up-regulation proteins.
Of the 183 identified proteins, 100 were shared between groups, totaling 57 up-regulated and 27 downregulated proteins in G1. Key biological processes included antimicrobial humoral response and hydrogen peroxide catabolism, with proteins linked to immune function and endopeptidase regulation. Functional analysis showed that Lactotransferrin (5-fold increase in G1), Haptoglobin (4-fold), and Immunoglobulin J chain (3-fold) were up-regulated, whereas Statherin (5-fold) and Protein S100-A8 (4-fold) were downregulated in G1.
Pregnant women with obesity and generalized gingivitis exhibited a distinct salivary proteomic profile characterized by the up-regulation of immune-related proteins and downregulation of tissue-protective proteins. These findings suggest potential salivary biomarkers for detection and targeted management of periodontal inflammation in this high-risk population.
本横断面研究调查了肥胖孕妇中与广泛性牙龈炎相关的唾液蛋白质组学特征。
将孕晚期（妊娠≥27周）的孕妇根据探诊出血情况（BOP）分为两组：G1组（BOP>50%；n=9）和G2组（BOP 0-30%；n=9）。收集未刺激的唾液样本，使用纳升液相色谱电喷雾电离串联质谱进行单独分析。根据基因本体论对鉴定出的蛋白质进行生物过程、分子功能、免疫系统参与情况和细胞成分分类。使用下调p<0.05和上调蛋白质1-p>0.95的阈值确定差异蛋白表达。
在鉴定出的183种蛋白质中，两组共有100种，G1组共有57种上调蛋白和27种下调蛋白。关键生物过程包括抗微生物体液反应和过氧化氢分解代谢，涉及与免疫功能和内肽酶调节相关的蛋白质。功能分析显示，乳铁蛋白（G1组增加5倍）、触珠蛋白（4倍）和免疫球蛋白J链（3倍）上调，而磷蛋白（5倍）和蛋白质S100-A8（4倍）在G1组中下调。
肥胖且患有广泛性牙龈炎的孕妇表现出独特的唾液蛋白质组学特征，其特点是免疫相关蛋白上调和组织保护蛋白下调。这些发现提示了在这一高危人群中检测和针对性管理牙周炎症的潜在唾液生物标志物。
