Ilyas A M, Alam Md Kowsar, Musah Jamal-Deen, Yang Mengsu, Roy Vellaisamy A L, Lam Yun Wah, Lau Condon
Department of Physics, City University of Hong Kong, Kowloon 999077, Hong Kong, China; Department of Physics, Federal University Oye-Ekiti, Ekiti State 3600001, Nigeria.
Department of Biomedical Sciences, City University of Hong Kong, Kowloon 999077, Hong Kong, China; Department of Physics, University of Chittagong, Chittagong 4331, Bangladesh.
Biosens Bioelectron. 2021 Jun 1;181:113142. doi: 10.1016/j.bios.2021.113142. Epub 2021 Mar 5.
Radiation-induced bystander effects (RIBE) have raised many concerns about radiation safety and protection. In RIBE, unirradiated cells receive signals from irradiated cells and exhibit irradiation effects. Until now, most RIBE studies have been based on morphological and biochemical characterization. However, research on the impact of RIBE on biophysical properties of cells has been lagging. Non-invasive indium tin oxide (ITO)-based impedance systems have been used as bioimpedance sensors for monitoring cell behaviors. This powerful technique has not been applied to RIBE research. In this work, we employed an electrical cell-ITO substrate impedance system (ECIIS) to study the RIBE on Chinese hamster ovary (CHO) cells. The bioimpedance of bystander CHO cells (BCHO), alpha(α)-particle (Am-241) irradiated CHO (ICHO), and untreated/unirradiated CHO (UCHO) cells were monitored with a sampling interval of 8 s over a period of 24 h. Media from ICHO cells exposed to different radiation doses (0.3 nGy, 0.5 nGy, and 0.7 nGy) were used to investigate the radiation dose dependence of BCHO cells' impedance. In parallel, we imaged the cells at times where impedance changes were observed. By analyzing the changes in absolute impedance and cell size/cell number with time, we observed that BCHO cells mimicked ICHO cells in terms of modification in cell morphology and proliferation rate. Furthermore, these effects appeared to be time-dependent and inversely proportional to the radiation dose. Hence, this approach allows a label-free study of cellular responses to RIBE with high sensitivity and temporal resolution and can provide crucial insights into the RIBE mechanism.
辐射诱导的旁观者效应（RIBE）引发了许多关于辐射安全与防护的担忧。在RIBE中，未受辐照的细胞会从受辐照细胞接收信号并表现出辐照效应。到目前为止，大多数RIBE研究都是基于形态学和生物化学特征。然而，关于RIBE对细胞生物物理特性影响的研究一直滞后。基于氧化铟锡（ITO）的非侵入式阻抗系统已被用作生物阻抗传感器来监测细胞行为。这项强大的技术尚未应用于RIBE研究。在这项工作中，我们采用细胞-ITO基底电阻抗系统（ECIIS）来研究对中国仓鼠卵巢（CHO）细胞的RIBE。以8秒的采样间隔在24小时内监测旁观者CHO细胞（BCHO）、α（α）粒子（Am-241）辐照的CHO（ICHO）和未处理/未受辐照的CHO（UCHO）细胞的生物阻抗。使用来自暴露于不同辐射剂量（0.3 nGy、0.5 nGy和0.7 nGy）ICHO细胞的培养基来研究BCHO细胞阻抗的辐射剂量依赖性。同时，我们在观察到阻抗变化的时间点对细胞进行成像。通过分析绝对阻抗和细胞大小/细胞数量随时间的变化，我们观察到BCHO细胞在细胞形态和增殖速率的改变方面模仿了ICHO细胞。此外，这些效应似乎是时间依赖性的，并且与辐射剂量成反比。因此，这种方法能够以高灵敏度和时间分辨率对细胞对RIBE的反应进行无标记研究，并可为RIBE机制提供关键见解。
