State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences/Guizhou Provincial Engineering Research Center for Natural Drugs, Guiyang 550014, China; Institute of Animal Husbandry and Veterinary Sciences, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China.
Ecotoxicol Environ Saf. 2021 Jun 1;215:112131. doi: 10.1016/j.ecoenv.2021.112131. Epub 2021 Mar 19.
The odor compound from Periploca sepium Bunge, 2-hydroxy-4-methoxy-benzaldehyde (HMB), is an allelochemical agent and is one of the least investigated isomers of vanillin. In this study, we used label-free quantitative proteomics analysis technology to investigate the effect of HMB on the protein expression of Humulus scandens (Lour.) Merr. leaves in July 2019 on Guiyang. A total of 269 proteins of 624 identified proteins were differentially expressed, among which 21.18% of the proteins were up-regulated and 32.71% down-regulated. These proteins were classified into 11 cell components and more than 20% of differentially expressed proteins were located in cell membrane and chloroplast. Functional classification analysis showed that 12 molecular functions were altered upon HMB treatment, and the ratio of catalytic activity was the highest (19.53%). At least 12 biological functions were affected, which involved small molecule metabolic processes, organic substance metabolic processes, gene expression, and photosynthesis. Our data provide resources and insights into the biochemical mechanism by which HMB kills weeds.
从白首乌中分离出的气味化合物 2-羟基-4-甲氧基苯甲醛（HMB）是一种化感物质，也是香草醛最少研究的异构体之一。本研究采用无标记定量蛋白质组学分析技术，研究了 HMB 对 2019 年 7 月贵阳葎草叶片蛋白质表达的影响。在鉴定的 624 种蛋白质中，有 269 种蛋白质差异表达，其中 21.18%的蛋白质上调，32.71%下调。这些蛋白质被分为 11 种细胞成分，超过 20%的差异表达蛋白位于细胞膜和叶绿体中。功能分类分析表明，HMB 处理后 12 种分子功能发生改变，催化活性最高（19.53%）。至少有 12 种生物功能受到影响，涉及小分子代谢过程、有机物质代谢过程、基因表达和光合作用。我们的数据为 HMB 除草的生化机制提供了资源和见解。
