Faculty of Agriculture, Department of Agricultural Biotechnology, Division of Animal Biotechnology, Akdeniz University, Antalya, Turkey.
Faculty of Agriculture, Department of Agricultural Biotechnology, Ondokuz Mayıs University, Samsun, Turkey.
J Enzyme Inhib Med Chem. 2021 Dec;36(1):885-894. doi: 10.1080/14756366.2021.1900167.
Here we investigated the effects of different levels of royal jelly in zebrafish () diets [0.0% (D1); 0.1% (D2); 0.4% (D3); 1.6% (D4) vs 6.4% (D5)] on the activity and expression profiles of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase and glutathione S-transferase. Muscle, liver and kidney tissue samples were obtained from fish fed during 8 weeks. In these tissues, enzyme activity was determined by means of spectrophotometer and gene expression by quantitative real-time PCR. mRNA levels of the enzymes were elevated in almost all diet groups compared to the control (D1). It was determined that enzyme activities were also increased in general by supplementation of royal jelly although some decreases were also observed. However, the significant correlation between gene expression and enzyme activity was not observed in all tissues. It was concluded that main regulation occurs with post-translational modifications although effects at transcriptomic level demonstrated a snap variation.
在这里，我们研究了不同水平的蜂王浆在斑马鱼饮食中的作用[0.0%（D1）；0.1%（D2）；0.4%（D3）；1.6%（D4）与 6.4%（D5）]对超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶、谷胱甘肽过氧化物酶和谷胱甘肽 S-转移酶的活性和表达谱的影响。在 8 周的喂养期间，从喂食的鱼中获得肌肉、肝脏和肾脏组织样本。通过分光光度计测定这些组织中的酶活性，通过定量实时 PCR 测定基因表达。与对照组（D1）相比，几乎所有饮食组的酶 mRNA 水平均升高。结果表明，尽管也观察到一些下降，但蜂王浆的补充通常会增加酶活性。然而，在所有组织中均未观察到基因表达与酶活性之间的显著相关性。结论是主要的调节发生在翻译后修饰上，尽管转录组水平的影响表现出瞬时变化。
