Freihorst J, Piedra P A, Okamoto Y, Ogra P L
Department of Pediatrics, State University of New York, Buffalo 14222.
Proc Soc Exp Biol Med. 1988 Jun;188(2):191-7. doi: 10.3181/00379727-188-42727.
Groups of BALB/c mice were sham infected or inoculated intranasally (IN) with live RSV. From Day 4 to 8 after infection, the animals were exposed IN to ovalbumin (OVA) with or without alum adjuvant. At different intervals, levels of OVA concentration in serum, IgG-anti-OVA antibody activity in serum, and IgA-anti-OVA antibody activity in bronchial washings were determined, employing the ELISA technique. IgE-anti-OVA antibody titers in serum and bronchial washings were assessed by PCA. OVA concentrations in serum were significantly higher in RSV-infected animals compared to uninfected controls. The use of alum adjuvant also increased OVA uptake in uninfected animals but to a lesser extent than RSV infection. RSV-infected animals developed significantly higher OVA-specific antibody titers of IgG isotype in serum and IgA isotype in bronchial washings than the uninfected controls, while alum enhanced the immune response less markedly but still significantly in uninfected mice. An IgE antibody response to OVA in serum was demonstrable in 50% of RSV-infected mice immunized IN with OVA and alum, while all uninfected animals and RSV-infected animals immunized with OVA alone (without adjuvant) failed to develop a detectable IgE response. These findings suggest that infections with viral agents such as RSV may function as adjuvants for other antigens inhaled during acute respiratory infection. These observations may explain the alterations in the immune response to other antigens in patients with acute viral-induced bronchopulmonary diseases.
将BALB/c小鼠分为几组，分别进行假感染或经鼻内（IN）接种活的呼吸道合胞病毒（RSV）。在感染后的第4天至第8天，将动物经鼻内暴露于卵清蛋白（OVA），添加或不添加明矾佐剂。在不同时间间隔，采用酶联免疫吸附测定（ELISA）技术测定血清中OVA浓度水平、血清中IgG抗OVA抗体活性以及支气管灌洗液中IgA抗OVA抗体活性。通过被动皮肤过敏试验（PCA）评估血清和支气管灌洗液中IgE抗OVA抗体滴度。与未感染的对照组相比，RSV感染动物血清中的OVA浓度显著更高。使用明矾佐剂也增加了未感染动物对OVA的摄取，但程度低于RSV感染。RSV感染动物血清中IgG同型和支气管灌洗液中IgA同型的OVA特异性抗体滴度显著高于未感染的对照组，而明矾在未感染小鼠中对免疫反应的增强作用虽不那么明显但仍显著。在用OVA和明矾经鼻内免疫的RSV感染小鼠中，50%的小鼠血清中可检测到对OVA的IgE抗体反应，而所有未感染动物以及仅用OVA（无佐剂）免疫的RSV感染动物均未产生可检测到的IgE反应。这些发现表明，诸如RSV等病毒感染可能作为急性呼吸道感染期间吸入的其他抗原的佐剂。这些观察结果可能解释了急性病毒诱发的支气管肺部疾病患者对其他抗原免疫反应的改变。
