Beck L, Spiegelberg H L
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Cell Immunol. 1989 Oct 1;123(1):1-8. doi: 10.1016/0008-8749(89)90263-3.
BALB/c mice were injected ip with 1 microgram ovalbumin (OVA) in alum or complete Freund's adjuvant (cFA) and the changes of the IgE and IgG subclass serum levels and isotypes of the anti-OVA specific antibodies determined by radioimmunoassays. By Day 10, OVA in alum had induced a 5- to 10-fold increase of the IgE serum level and an initial decrease of the IgG subclass levels which subsequently increased to two to threefold over the preinjection level. OVA in cFA induced a gradual twofold increase of the IgE serum level, a rapid fourfold increase of the IgG2a level occurring by Day 7, and a gradual two to threefold increase of the other IgG subclasses. Over 90% of the anti-OVA antibodies were of the IgGl isotype with both adjuvants; OVA in alum induced slightly more IgGl anti-OVA antibodies than cFA. In contrast, the OVA in alum injected mice formed significantly more (5- to 10-fold) IgE anti-OVA antibodies than the cFA-injected mice. OVA in alum also induced a large nonspecific increase of the IgE serum level because only approximately 40% of the increase observed on Day 14 was absorbable with OVA, whereas approximately 90% the IgE increase in cFA injected mice was absorbable with OVA. The data demonstrate that mice form mainly IgGl and IgE antibodies to OVA irrespective of the adjuvant. The low specific and lack of nonspecific IgE formation by mice injected with OVA in cFA may be the result of cFA-induced interferon-gamma (IFN-gamma) production because IFN-gamma has been shown to stimulate IgG2a and inhibit IgE secretion in vitro.
给BALB/c小鼠腹腔注射1微克卵清蛋白（OVA）与明矾或完全弗氏佐剂（cFA）的混合物，通过放射免疫测定法测定抗OVA特异性抗体的IgE和IgG亚类血清水平及同种型的变化。到第10天，与明矾混合的OVA使IgE血清水平升高了5至10倍，IgG亚类水平最初下降，随后升高至注射前水平的两至三倍。与cFA混合的OVA使IgE血清水平逐渐升高两倍，IgG2a水平在第7天迅速升高四倍，其他IgG亚类逐渐升高两至三倍。两种佐剂诱导产生的抗OVA抗体中，超过90%为IgG1同种型；与明矾混合的OVA诱导产生的IgG1抗OVA抗体略多于cFA。相比之下，注射了与明矾混合的OVA的小鼠产生的IgE抗OVA抗体比注射cFA的小鼠显著更多（5至10倍）。与明矾混合的OVA还诱导IgE血清水平大幅非特异性升高，因为在第14天观察到的升高中只有约40%可被OVA吸收，而注射cFA的小鼠中约90%的IgE升高可被OVA吸收。数据表明，无论使用何种佐剂，小鼠针对OVA主要产生IgG1和IgE抗体。注射与cFA混合的OVA的小鼠产生的特异性IgE低且无IgE非特异性产生，可能是cFA诱导产生干扰素-γ（IFN-γ）的结果，因为已证明IFN-γ在体外可刺激IgG2a并抑制IgE分泌。
